Anti nectin 2 cd112 apc

Manufactured by R&D Systems

Anti-Nectin-2/CD112-APC is a fluorescently-labeled antibody that binds to the Nectin-2/CD112 cell surface protein. Nectin-2/CD112 is a member of the immunoglobulin superfamily and functions in cell-cell adhesion. The APC fluorescent label allows for the detection of Nectin-2/CD112-expressing cells using flow cytometry.

Automatically generated - may contain errors

Lab products found in correlation

Bicinchoninic acid assay, by Thermo Fisher Scientific (4 mentions) Anti ulbp1 pe, by BD (2 mentions) Anti trail r2 apc, by R&D Systems (2 mentions) Anti cd155 pvr pe, by R&D Systems (2 mentions) Anti mycn, by Santa Cruz Biotechnology (1 mentions) Anti mica, by Abcam (1 mentions) Anti p53, by Santa Cruz Biotechnology (1 mentions) Facscanto 2, by BD (1 mentions) Apc conjugated annexin 5, by BD (1 mentions) Anti p53 fl393, by Santa Cruz Biotechnology (1 mentions) Anti cd45 hi30, by BD (1 mentions) Anti mica, by R&D Systems (1 mentions) Anti actin 1 19, by Santa Cruz Biotechnology (1 mentions) Anti ulbp2 5 6 pe, by R&D Systems (1 mentions) Anti ulbp3 pe, by R&D Systems (1 mentions) Cellrox deep red reagent, by Thermo Fisher Scientific (1 mentions) Propidium iodide, by Merck Group (1 mentions) Facsdiva software, by BD (1 mentions)

2 protocols using anti nectin 2 cd112 apc

Comprehensive Antibody Characterization for Western Blot and Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used: anti-MYCN, anti-p53, anti-Actin (B8.4.B, FL-393 and I-19, respectively, Santa Cruz Biotechnology), anti-MYC (Y69, OriGene), and anti-MDM2 (2A10, Calbiochem-Millipore) for western blotting; anti-CD107a-FITC (H4A3), anti-CD3-Alexa-700 (UCHT1), anti-CD56-PE-Cy7 (B159), anti-CD45 (HI30), FITC-conjugated rat anti-mouse IgG1 (A85–1) and PE-conjugated rat anti-mouse IgM (R6–60.2) purchased from BD Biosciences; anti-ULBP1-PE (170818), anti-ULBP2/5/6-PE (165903), anti-ULBP3-PE (166510), anti-MICA (159227), anti-MICB (236511), anti-TRAIL/R2-APC (17908), anti-CD155/PVR-PE (300907), anti-Nectin-2/CD112-APC (610603) purchased from R&D Systems; W6/32 which recognizes human fully-assembled MHC class I heavy chains and goat F(ab’)2 Fragment anti-mouse IgG FITC (IM1619, Dako) for flow cytometry; anti-MICA and anti-Nectin-2 (62540 and 154895, respectively, Abcam) for immunohistochemistry assay.
Whole-cell extracts were quantified by the bicinchoninic acid assay (Thermo Fisher Scientific), resolved on 8–10% SDS-PAGE and electroblotted. Filters were probed with primary antibodies followed by goat anti-mouse IgG HRP conjugated (Jackson). Flow cytometry was performed on FACSCantoII (BD Bioscences) and analyzed by FlowJo Software.

Brandetti E., Veneziani I., Melaiu O., Pezzolo A., Castellano A., Boldrini R., Ferretti E., Fruci D., Moretta L., Pistoia V., Locatelli F, & Cifaldi L. (2017). MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma. Oncoimmunology, 6(6), e1316439.

+ Open protocol

+ Expand

Immune Profiling of NB Tumor Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies for flow cytometry were used: anti-CD107a-FITC (H4A3), anti-CD3-Alexa-700 (UCHT1), anti-CD56-PE-Cy7 (B159), and anti-CD45 (HI30), purchased from BD Biosciences; anti-ULBP1-PE (170818), anti-ULBP2/5/6-PE (165903), anti-ULBP3-PE (166510), anti-MICA (159227), anti-MICB (236511), anti-TRAIL/R2-APC (17908), anti-CD155/PVR-PE (300907), and anti-Nectin-2/CD112-APC (610603), purchased from R&D Systems; W6/32 which recognizes human fully assembled MHC class I heavy chains; and goat F(ab′)2 Fragment anti-mouse IgG FITC (IM1619, Dako) for flow cytometry. Apoptosis of tumor cells was evaluated with APC-conjugated AnnexinV (BD-Pharmingen) and propidium iodide (PI) (Sigma-Aldrich).
Flow cytometry was performed on FACSCantoII and analysed by FACSDiva Software (BD Biosciences).
ROS production was evaluated in drug-treated NB cell lines by using CellROX Deep Red Reagent (C10422, Invitrogen) and measured by flow cytometry.
Whole-cell extracts were quantified by a bicinchoninic acid assay (Thermo Fisher Scientific), resolved on 8–10% SDS-PAGE and electroblotted. Filters were probed with primary antibodies followed by goat anti-mouse and HRP-conjugated rabbit anti-goat IgG (Jackson). The following antibodies for Western blotting were used: anti-p53 (FL-393) and anti-actin (I-19), purchased by Santa Cruz Biotechnology.

Veneziani I., Brandetti E., Ognibene M., Pezzolo A., Pistoia V, & Cifaldi L. (2018). Neuroblastoma Cell Lines Are Refractory to Genotoxic Drug-Mediated Induction of Ligands for NK Cell-Activating Receptors. Journal of Immunology Research, 2018, 4972410.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!