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Alexa fluor 647 tagged ova

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Alexa Fluor 647-tagged OVA is a fluorescent-labeled ovalbumin protein. Alexa Fluor 647 is a far-red fluorescent dye that can be used for various applications, such as flow cytometry, microscopy, and in vitro assays.

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Lab products found in correlation

Streptavidin bb515, by BD (2 mentions) Foxp3 transcription factor buffer set, by Thermo Fisher Scientific (2 mentions) Fc block 2.4g2, by BD (2 mentions) Np phycoerytherin pe, by Biosearch Technologies (2 mentions)

Close spelling variants of this product

Alexa Fluor 647 (1520 citations) Alexa 647 (424 citations) Alexa Fluors (21 citations) OVA-Alexa Fluor 647 (14 citations)

2 protocols using alexa fluor 647 tagged ova

1

Multiparametric Flow Cytometry Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Spleens were forced through a 70-μm strainer into PBS supplemented with 5% fetal calfserum (FCS). Red blood cells(RBCs)werelysed withACKbuffer(Gilbco) followed by 10-min incubation with 1 μg/mL Fc Block (24G2; BD Biosciences), and then stained for 30 min at 4°C with the following antibodies acquired from Biolegend or BD Biosciences: B220 (RA3-6B2), CD138 (281-2), CD19 (ID3), CD38 (90), CD4 (RM4-5), CD86 (GL-1), CXCR5 (2G8), CXCR4 (2B11), Foxp3 (MF-23), GL-7 (GL7), ICOS (7E.17G9), IgD (11-26c.2a), IgG1 (A85-1), and PD-1 (J43). Antigen-specific GC responses were detected with Alexa Fluor 647-tagged OVA (Thermo Fisher), NP-phycoerytherin (PE) (Biosearch Technologies), or with FITC-tagged CRM (made in house) amplified by biotinylated α-FITC (Biolegend) and BB515 streptavidin (BD Biosciences). Phospho-S6 (pS6) staining was performed as described by Ersching et al. (2017) (link). For intracellular staining, samples were fixed and permeabilized prior to staining (Foxp3/transcription factor buffer set; eBioscience). Acquisition was conducted on a Cytek DxP11 FACSCalibur and analyzed on FlowJo X (Tree Star).
Silva M., Nguyen T.H., Philbrook P., Chu M., Sears O., Hatfield S., Abbott R.K., Kelsoe G, & Sitkovsky M.V. (2017). Targeted Elimination of Immunodominant B Cells Drives the Germinal Center Reaction toward Subdominant Epitopes. Cell reports, 21(13), 3672-3680.
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2

Multiparametric Flow Cytometry Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Spleens were forced through a 70-μm strainer into PBS supplemented with 5% fetal calfserum (FCS). Red blood cells(RBCs)werelysed withACKbuffer(Gilbco) followed by 10-min incubation with 1 μg/mL Fc Block (24G2; BD Biosciences), and then stained for 30 min at 4°C with the following antibodies acquired from Biolegend or BD Biosciences: B220 (RA3-6B2), CD138 (281-2), CD19 (ID3), CD38 (90), CD4 (RM4-5), CD86 (GL-1), CXCR5 (2G8), CXCR4 (2B11), Foxp3 (MF-23), GL-7 (GL7), ICOS (7E.17G9), IgD (11-26c.2a), IgG1 (A85-1), and PD-1 (J43). Antigen-specific GC responses were detected with Alexa Fluor 647-tagged OVA (Thermo Fisher), NP-phycoerytherin (PE) (Biosearch Technologies), or with FITC-tagged CRM (made in house) amplified by biotinylated α-FITC (Biolegend) and BB515 streptavidin (BD Biosciences). Phospho-S6 (pS6) staining was performed as described by Ersching et al. (2017) (link). For intracellular staining, samples were fixed and permeabilized prior to staining (Foxp3/transcription factor buffer set; eBioscience). Acquisition was conducted on a Cytek DxP11 FACSCalibur and analyzed on FlowJo X (Tree Star).
Silva M., Nguyen T.H., Philbrook P., Chu M., Sears O., Hatfield S., Abbott R.K., Kelsoe G, & Sitkovsky M.V. (2017). Targeted Elimination of Immunodominant B Cells Drives the Germinal Center Reaction toward Subdominant Epitopes. Cell reports, 21(13), 3672-3680.
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