Gene fragments

Manufactured by Thermo Fisher Scientific

Gene fragments are synthetic nucleic acid sequences that represent specific regions or portions of a gene. They are used as building blocks in various molecular biology and genetic engineering applications.

Automatically generated - may contain errors

Lab products found in correlation

Hek293f cell, by Thermo Fisher Scientific (1 mentions) Gibson assembly, by Thermo Fisher Scientific (1 mentions) Pdonr221, by Thermo Fisher Scientific (1 mentions) Gateway lr clonase 2 system, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Synthetic gene fragment (1 citations)

2 protocols using gene fragments

Engineered SARS-CoV-2 Spike Variants

Check if the same lab product or an alternative is used in the 5 most similar protocols

Pre-fusion spike protein ectodomain DNA constructs were designed containing the following mutations compared to the Wuhan variant (Wuhan Hu-1; GenBank: MN908947.3): deletion of H69, V70 and Y144, N501Y, A570D, D614G, P681H, T716I, S982A and D1118H in Alpha; L18F, D80A, D215G, L242H, R246I, K417N, E484K, N501Y, D614G and A701V in Beta; L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y and T1027I in Gamma. The constructs were ordered as gene fragments (Integrated DNA Technologies) and inserted into a pPPI4 expression vector containing a hexahistidine (his) tag using Gibson Assembly (ThermoFisher). All spike constructs were produced in HEK293F cells (ThermoFisher) and purified using NiNTA chromatography and size exclusion chromatography as previously described^{58 (link)}. The Delta spike protein was provided by Dirk Eggink and Chantal Reusken (National Institute for Public Health and the Environment, the Netherlands).

van der Velden Y.U., Grobben M., Caniels T.G., Burger J.A., Poniman M., Oomen M., Rijnstra E.S., Tejjani K., Guerra D., Kempers R., Stegmann T., van Gils M.J, & Sanders R.W. (2022). A SARS-CoV-2 Wuhan spike virosome vaccine induces superior neutralization breadth compared to one using the Beta spike. Scientific Reports, 12, 3884.

+ Open protocol

+ Expand

Cloning Cbx-KRAB Domain Fusions

Check if the same lab product or an alternative is used in the 5 most similar protocols

Genes encoding Cbx-KRAB domain fusions were synthesized as gene fragments (Integrated DNA Technologies) and cloned with the Gateway BP Clonase II system (Thermo Fisher, 11789020) into the Gateway Entry vector pDONR221 (Thermo Fisher) according to the manufacturer’s protocols. Cbx-KRAB domain fusions cloned into pDONR221 were then transferred, via Gateway LR Clonase II system (Thermo Fisher, 11791020), into the pLX303-dCas9 vector^{38 (link)} (a kind gift of Dr. Taipale, University of Toronto). The pLX303-dCas9 vector encoded a Streptococcus pyogenes dCas9 with two C-terminal and one N-terminal SV40 nuclear localization signals. See Supplementary Dataset 5 for sequences of all dCas9-repressors used in this study.

Veggiani G., Villaseñor R., Martyn G.D., Tang J.Q., Krone M.W., Gu J., Chen C., Waters M.L., Pearce K.H., Baubec T, & Sidhu S.S. (2022). High-affinity chromodomains engineered for improved detection of histone methylation and enhanced CRISPR-based gene repression. Nature Communications, 13, 6975.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!