Dpx new mounting medium

Animals were fixed in Karnovsky fixative, washed twice in PBS, dehydrated in a graded ethanol series, pre-infiltrated with Technovit7100/ethanol (50/50) for 4 hours (Technovit 7100, Heraeus Kulzer Technique) and infiltrated with Technovit7100/hardener overnight before embedding in plastic. Two μm thick sections were produced using a microtome (Leica RM 2165) and stained with toluidine blue (1% toluidine blue in 2% borax) for 30 s. The stained sections were mounted using DPX New Mounting Medium (Merck). Photographs were taken using a Zeiss Axicam 105 color (Zeiss) camera mounted to Zeiss Axio Scope.A1 (Zeiss) microscope.

L. salmonis were fixed in Karnovsky fixative with 4% sucrose overnight at 4 °C. Thereafter, lice were washed twice in 1% PBS, dehydrated once in 75% ethanol, and twice in 96% ethanol for 15 min. They were then pre-infiltrated with Technovit 7100/ethanol (50/50) for two hours (Technovit 7100, Kulzer, Heraeus, Germany) and infiltrated with Technovit 7100/hardener overnight before embedding in plastic. The embedded lice were cut into 2 μm thick sections, then dried at 50 °C before they were stained with toluidine blue (1% toluidine blue in 2% borax). The slides were stained for 30 s in the staining solution, then washed in running tap water. The stained sections were mounted with DPX New Mounting Medium (Merck, Darmstadt, Germany). Photos were taken using a Zeiss Axicam 105 color (Zeiss) camera attached to a Zeiss Axio Scope.A1 (Zeiss) microscope.