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C2 confocal fluorescence microscope

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The Nikon C2 is a confocal fluorescence microscope designed for high-resolution imaging and analysis of samples. It features a laser-scanning system that allows for the capture of detailed, optical sections of specimens. The C2 is capable of acquiring images with a high signal-to-noise ratio and minimal background interference, making it a versatile tool for a wide range of applications in research and scientific fields.

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Lab products found in correlation

Alexa fluor 568, by Thermo Fisher Scientific (2 mentions) Prolong diamond antifade mountant with 4 6 diamidino 2 phenylindol dapi, by Thermo Fisher Scientific (2 mentions) Superfrost microscope slide, by Thermo Fisher Scientific (1 mentions) Dpx mounting medium, by Merck Group (1 mentions) Ig alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Vectashield, by Vector Laboratories (1 mentions)

Spelling variants of this product

Fluorescence microscope (1854 citations) Confocal microscope (523 citations) C2 confocal microscope (379 citations) Confocal fluorescence microscope (57 citations) C2 microscope (34 citations) C2 confocal (20 citations) C2 fluorescence microscope (2 citations)

4 protocols using c2 confocal fluorescence microscope

1

Fluoro-Jade C Staining of Mouse Brain Tissue

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4-month-old mouse brain tissues were first embedded in paraffin, and then trimmed and cut into 5 μm thickness sections before being mounted on SuperFrost microscope slides (Thermo Fisher Scientific, NH, USA). Afterward, tissue sections were deparaffinized and rehydrated under a gradient xylene-ethanol procedure. First, slides were immersed in 1% NaOH diluted in 80% ethanol for 2 min, then rinsed in 70% ethanol for 2 min, followed by distilled water-rinsing for another 2 min. After the rinse, the slides were incubated in 0.06% potassium permanganate for 10 min. Following another 2-min rinse in water, slides were then transferred into a solution containing 0.0001% Fluoro-Jade C in 0.1% acetic acid and then incubated for another 10 min. After washing three times with water (2 min per wash), slides were dried in an incubator at 50°C for 30 min, then coverslipped with DPX mounting medium (Sigma-Aldrich, MO, USA) after being cleared in xylene. Signals were visualized using a Nikon C2 confocal fluorescence microscope.
Yang L., Slone J., Zou W., Queme L.F., Jankowski M.P., Yin F, & Huang T. (2020). Systemic Delivery of AAV-Fdxr Mitigates the Phenotypes of Mitochondrial Disorders in Fdxr Mutant Mice. Molecular Therapy. Methods & Clinical Development, 18, 84-97.
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2

Immunofluorescence Localization of IKAROS

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Cytospins of mouse pre-B cells and cryopreserved Epstein-Barr virus (EBV)-transformed lymphocytes from members of the index family, were fixed with 4% paraformaldehyde and washed with phosphate-buffered saline (PBS), followed by a 30 min incubation in a blocking/permeabilization solution of 10% normal goat serum (NGS)/0.1% Triton-X 100/PBS, and then incubated for 1 hr with primary anti-IKAROS antibody (H-100, Santa Cruz Biotechnology, Santa Cruz, CA) diluted in 3% NGS/0.1% Triton-X 100/PBS. Slides were washed three times in PBS, and then incubated for 45 min in 3% NGS/0.1% Triton-X 100/PBS containing a secondary antibody conjugated to Ig-Alexa Fluor 488 (Invitrogen, Carlsbad, CA). Slides were washed three times in PBS and mounted with Vectashield (Vector Labs, Burlingame, CA) containing 4′-6-diamidino-2-phenylindol (DAPI). All steps were carried out at room temperature. Images were captured using a Nikon C2 confocal fluorescence microscope and analyzed for nuclear versus cytoplasmic localization using NIS Elements software.
Churchman M.L., Qian M., te Kronnie G., Zhang R., Yang W., Zhang H., Lana T., Tedrick P., Baskin R., Verbist K., Peters J.L., Devidas M., Larsen E., Moore I.M., Gu Z., Qu C., Yoshihara H., Porter S.N., Pruett-Miller S.M., Wu G., Raetz E., Martin P.L., Bowman W.P., Winick N., Mardis E., Fulton R., Stanulla M., Evans W.E., Relling M.V., Pui C.H., Hunger S.P., Loh M.L., Handgretinger R., Nichols K.E., Yang J.J, & Mullighan C.G. (2018). Germline Genetic IKZF1 Variation and Predisposition to Childhood Acute Lymphoblastic Leukemia. Cancer cell, 33(5), 937-948.e8.
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3

Immunofluorescence Analysis of ITGB1 Expression

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Cytospins of NALM6 alone and co-cuture samples of NALM6 with BM-MSC were fixed with 4% paraformaldehyde and washed with PBS, followed by 1 h incubation in a 3% BSA blocking buffer. After removal of blocking buffer, Cells were permeabilized by incubation with 0.1% Triton X-100 in PBS for 10 min at room temperature and then incubated for 1 h with primary anti-ITGB1 antibody (P5D2, Abcam) diluted in 3% NGS/0.1% Triton X-100/PBS. Slides were washed three times in PBS, and then incubated for 1 h in 3% NGS/0.1% Triton X-100/PBS containing goat anti-rabbit Alexa Fluor 568 (Invitrogen, Carlsbad, CA). Slides were washed three times in PBS and mounted with ProLong Diamond Antifade Mountant with 4′-6-diamidino-2-phenylindol (DAPI) (ThermoFisher Scientific). All steps were carried out at room temperature. Images were captured using a Nikon C2 confocal fluorescence microscope and analyzed using NIS Elements software.
Park C.S., Yoshihara H., Gao Q., Qu C., Iacobucci I., Ghate P.S., Connelly J.P., Pruett-Miller S.M., Wagner B., Robinson C.G., Mishra A., Peng J., Yang L., Rankovic Z., Finkelstein D., Luger S., Litzow M., Paietta E.M., Hebbar N., Velasquez M.P, & Mullighan C.G. (2023). Stromal-induced epithelial-mesenchymal transition induces targetable drug resistance in acute lymphoblastic leukemia. Cell reports, 42(7), 112804.
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4

Immunofluorescence Analysis of ITGB1 Expression

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Cytospins of NALM6 alone and co-cuture samples of NALM6 with BM-MSC were fixed with 4% paraformaldehyde and washed with PBS, followed by 1 h incubation in a 3% BSA blocking buffer. After removal of blocking buffer, Cells were permeabilized by incubation with 0.1% Triton X-100 in PBS for 10 min at room temperature and then incubated for 1 h with primary anti-ITGB1 antibody (P5D2, Abcam) diluted in 3% NGS/0.1% Triton X-100/PBS. Slides were washed three times in PBS, and then incubated for 1 h in 3% NGS/0.1% Triton X-100/PBS containing goat anti-rabbit Alexa Fluor 568 (Invitrogen, Carlsbad, CA). Slides were washed three times in PBS and mounted with ProLong Diamond Antifade Mountant with 4′-6-diamidino-2-phenylindol (DAPI) (ThermoFisher Scientific). All steps were carried out at room temperature. Images were captured using a Nikon C2 confocal fluorescence microscope and analyzed using NIS Elements software.
Galambos R., Szabó-Salfay O., Szatmári E., Szilágyi N, & Juhász G. (2001). Sleep modifies retinal ganglion cell responses in the normal rat. Proceedings of the National Academy of Sciences of the United States of America, 98(4), 2083-2088.
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