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2 protocols using prevotella nigrescens

1

Antibacterial Evaluation of Essential Oils

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The tested strains were obtained from the American Type Culture Collection (ATCC, Rockville MD, USA). The following microorganisms were used in the evaluation of the antibacterial activity of the essential oils: Streptococcus mitis (ATCC 49456), Streptococcus mutans (ATCC 25175), Streptococcus sanguinis (ATCC 10556), Streptococcus sobrinus (ATCC 33478), Actinomyces naeslundii (ATCC 19039), Bacteroides fragilis (ATCC 25285), and Prevotella nigrescens (ATCC 33563).
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2

Clostridium Strains Cultivation and Maintenance

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Clostridium difficile strain 630 (ATCC® BAA-1382-FZTM), Clostridium difficile strain 4118 (ATCC® BAA-1870TM), Clostridium sporogenes (ATCC® 15579TM), Clostridium clostridioforme (ATCC® 25537TM), Bacteroides thetaiotaomicron (ATCC® 29148TM), Parabacteroides distasonis (ATCC® 8503TM) Prevotella nigrescens (ATCC® 33563TM), and Bacillus subtilis (ATCC® 6051TM) were purchased from ATCC (Manassas, VA, USA). Pseudomonas aeruginosa PAO1 was provided by Dr. Kendra Rumbaugh. Bacteroides fragilis strain CL05T00C42, Bifidobacterium longum strain 44B, Bifidobacterium breve strain EX336960VC19, Lactobacillus reuteri strain CF48-3A, Lactobacillus johnsonii strain 135-1-CHN, and all other C. difficile isolates described in this study were from BEI Resources, NIAID, NIH.
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