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Ab10466

Manufactured by Abcam
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Ab10466 is a laboratory equipment product. It serves a core function, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

Automatically generated - may contain errors

Lab products found in correlation

Ab133458, by Abcam (1 mentions) Ab176560, by Abcam (1 mentions) Alkaline phosphatase conjugated goat anti mouse a3562 igg, by Merck Group (1 mentions) Alkaline phosphatase conjugated goat anti guinea pig igg, by Merck Group (1 mentions) Ab9485, by Abcam (1 mentions)

2 protocols using ab10466

1

Antibody Resources for Protein Analysis

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Antibodies against CK2 (ab10466), tubulin (ab176560), AKT1 pSer129 (ab133458) and CDC37 pSer13 (ab108360) were from Abcam. Antibodies against GAPDH (#2118), Lamin A/C (#2032), UBE2N (#6999), CDC37 (#3618) and AKT1 (#9272) were from Cell Signaling Technologies. The antibody against 53BP1 was from Bethyl Laboratories. The antibody against ubiquitin was from Dako (Z0458) and that against HA was from Roche (12CA5). HRP-conjugated antibodies against FLAG (#A8592) and HA (#12013819001) were from Sigma and Roche, respectively. Human recombinant full length OTUB1, ALK5 (amino acids 142-172) polypeptide, and pSer16-OTUB1 (amino acids 10-22, KQEPLGSDSEGVN) polypeptide were used as immunogens to generate the respective antibodies. All recombinant proteins, plasmids and antibodies generated for the present study are available on request and described in further detail on our reagents website (https://mrcppureagents.dundee.ac.uk/).
Herhaus L., Perez-Oliva A.B., Cozza G., Gourlay R., Weidlich S., Campbell D.G., Pinna L.A, & Sapkota G.P. (2015). Casein kinase 2 (CK2) phosphorylates the deubiquitylase OTUB1 at Ser16 to trigger its nuclear localization. Science signaling, 8(372), ra35.
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2

SDS-PAGE and Western Blot Analysis

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SDS-PAGE gels were transferred via a semidry blotter to PVDF transfer membranes and blocked for 4 h with Tris-buffered saline containing 0.05% Tween 20 and 10% (w/v) milk powder. The following primary antibodies were used for the detection of: NS2 (guinea pig anti-NS2 serum), VP3 (rabbit anti-VP3 serum), β-Actin (monoclonal anti-β-Actin antibody produced in mouse, clone AC-74, A2228, Sigma-Aldrich), GAPDH (rabbit anti-GAPDH (ab9485, Abcam), anti-CKII α antibody (ab10466, Abcam). These were added to blocked membranes and incubated overnight at 4 °C. Secondary antibodies were an alkaline phosphatase-conjugated goat anti-guinea pig IgG (1:10,000, Sigma-Aldrich, A5062), goat anti-rabbit (A0418), and an alkaline phosphatase-conjugated goat anti-mouse (A3562) IgG (1:10,000, Sigma-Aldrich).
Mohl B.P, & Roy P. (2016). Cellular Casein Kinase 2 and Protein Phosphatase 2A Modulate Replication Site Assembly of Bluetongue Virus. The Journal of Biological Chemistry, 291(28), 14566-14574.
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