Log In Sign up
The largest database of trusted experimental protocols

Cd11c pe cy7 b ly6

Manufactured by BD
Visit Supplier

CD11c-PE-Cy7 (B-ly6) is a monoclonal antibody used in flow cytometry applications to detect and quantify CD11c-expressing cells. It is conjugated with PE-Cy7 fluorescent dye for multicolor analysis.

Automatically generated - may contain errors

Lab products found in correlation

Mp4 25d2, by BioLegend (1 mentions) Cd45ra fitc, by BioLegend (1 mentions) Il 4 fitc, by BioLegend (1 mentions) Cd11c apc efluor780, by Thermo Fisher Scientific (1 mentions) Cd3 percp cy5, by BD (1 mentions) Cd4 apc, by BD (1 mentions) Foxp3 pe, by Thermo Fisher Scientific (1 mentions) Cd80 fitc, by Thermo Fisher Scientific (1 mentions) Hla dr apc, by BD (1 mentions) Cd27 pe cy7, by Thermo Fisher Scientific (1 mentions) Btla pe, by BD (1 mentions) Ccr7 fitc, by BD (1 mentions) Lin1 fitc, by BD (1 mentions) Facscanto 2, by BD (1 mentions) Live dead aqua staining, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

CD11c-PE-Cy7 (65 citations) CD11c-PE (57 citations) CD11c (B-ly6, (9 citations)

2 protocols using cd11c pe cy7 b ly6

1

Flow Cytometry and Cell Sorting

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following mouse anti-human antibodies were used for flow cytometry and cell sorting: CD11c-APC-eFluor780 (BU15, eBioscience), CD11c-PE-Cy7 (B-ly6, BD Pharmingen), Lin1-FITC (SK7, 3G8, SJ25C1, L27, eMfP9, NCAM16.2, BD Biosciences), Lin1-APC (UCHT1, HCD14, 3G8, HIB19, 2H7, HCD56, BioLegend), CD123-PerCP-CyTM5.5 (7G3, BD Biosciences), HLA-DR-APC-eFluor780 (LN3, eBioscience), HLA-DR-APC (G46-6, BD), CD86-FITC (FUN-1, BD Biosciences), CD80-FITC (2D10.4, eBioscience), CD83-FITC (HB15e, BD Biosciences), CCR7-FITC (150503, BD Biosciences), BTLA-APC (MIH26, Biolegend), BTLA-PE (J168-540, BD Pharmingen), CD3-PerCp-Cy5.5 (SP34-2, BD Biosciences), CD4-PerCP-Cy5.5 (RPA-T4, eBioscience), CD4-APC (L200, BD Biosciences), CD27-PE-Cy7 (0323, eBioscience), CD45RA-FITC (HI100, Biolegend), CD27-PE-Cy7 (0323, eBioscience), CD45RA-FITC (HI100, Biolegend), Foxp3-PE (PCH101, eBioscience), IFN-α-FITC (MMHA-11, InterferonSource), IL-12-FITC (MHCIL1201, Invitrogen), IL-4-FITC (MP4-25D2, Biolegend), and IFN-γ-APC-eFluor780 (45.B3, eBioscience).
Zhang J.A., Lu Y.B., Wang W.D., Liu G.B., Chen C., Shen L., Luo H.L., Xu H., Peng Y., Luo H., Huang G.X., Wu D.D., Zheng B.Y., Yi L.L., Chen Z.W, & Xu J.F. (2020). BTLA-Expressing Dendritic Cells in Patients With Tuberculosis Exhibit Reduced Production of IL-12/IFN-α and Increased Production of IL-4 and TGF-β, Favoring Th2 and Foxp3+ Treg Polarization. Frontiers in Immunology, 11, 518.
+ Open protocol
+ Expand
2

Identification of Dermal Dendritic Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Emigrated dermal dendritic cells (DDCs) were distinguished from other immune cells by their forward and side scatter properties, in addition to high expression of CD11c and HLA-DR. The different DDC subsets were determined using the expression of CD1a, CD14 and CD141: CD141+CD14− (referred to as CD141+ DDCs), CD1ahighCD14− (referred to as LCs), CD1aintCD14− (referred to as CD1a+ DDCs) and CD1a−CD14+ (referred to as CD14+ DDCs). Antibodies used were HLA-DR-PerCP-ef710 (L243, eBioscience), CD11c-PE-Cy7 (B-Ly6, BD Pharmingen), CD80-BV650 (L307.4, BD Biosciences), CD1a-AF700 (HI149), CD141-BV421 (M80), lineage cocktail-APC (CD3/19/20/56; Biolegend), CD14-PE-Texas Red (Tuk4, Life Technologies), PDL1-APC (MIH1) and PDL2-PE or PECy7 (MIH18; eBioscience). All conditions were incubated with CD16/32 Fc receptor inhibitor (eBioscience) and Aqua live/dead staining (Invitrogen). Samples were measured using a FACS canto-II (BD Bioscience Franklin Lakes, NJ, USA) and analyzed in FlowJoTM (FlowJo LLC, Ashland, OR, USA).
Winkel B.M., Dalenberg M.R., de Korne C.M., Feijt C., Langenberg M.C., Pelgrom L., Ganesh M.S., Yazdanbakhsh M., Smits H.H., de Jong E.C., Everts B., van Leeuwen F.W., Hokke C.H, & Roestenberg M. (2018). Early Induction of Human Regulatory Dermal Antigen Presenting Cells by Skin-Penetrating Schistosoma Mansoni Cercariae. Frontiers in Immunology, 9, 2510.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!