Images were taken on confocal laser scanning microscopes (Leica SP5, Leica SP8, Nikon A1Rsi+, Nikon Spinning Disk Confocal CSA-W1 SoRa), or on a widefield microscope (Nikon Widefield Ti2). Images were obtained with a 10× air, and 20×-, 40×-, or 63× oil immersion objectives (Leica: HCX PL APO 20×/0.7, HCX PL APO 63×/1.20W motCORR CS; Nikon 20× Plan Apo, Air, 0.8 NA, 1.000 DIC N2 VC, Nikon 40× Plan Flour, Oil, 1.3 NA, 200WD, DIC N2 H; Nikon 40× Plan Flour, Sil, 1.25 NA, 300WD, SR HP DIC N1 λS OFN25; Nikon 10× Plan Fluo Air, 0.3 NA, 15.200 WD, Ph 1 DL; Nikon 10× Plan Fluo Air, 0.45 NA, 4.000 WD, DIC N1 λ OFN25). Fast blue was imaged with a 405 nm, GFP with a 488 nm, and tdTom, mCherry, or RFP with a 561 nm laser (bandwidth, Leica, 425/70, 515/25, 590/70; Nikon, 447/60, 450/50, 525/50, 595/50; Nikon widefield, 435/33, 519/26, 595/31).
+ Open protocol