Cells were lysed in HEPES lysis buffer (50 mM HEPES, 150 mM NaCl, 2 mM EDTA, 1% NP40 and 0.5% sodium deoxycholate) or RIPA buffer (20 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA pH 8, 1% TRITON-X and 0.5% SDS) supplemented with 1 mM PMSF, 1 mM Na3VO4, 5 μg/ml leupeptin and 1 mM DTT and analysed by immunoblotting using antibodies against p-STAT1, p-STAT3, STAT1, HA (Cell Signaling Technology), STAT2, STAT3, p65 (Santa Cruz Biotechnologies), HIV-Vif (Abcam), p24 (NIH AIDS program) and β-actin (Sigma) and HRP-linked secondary anti-mouse or anti-rabbit antibodies (Invitrogen) and visualised using Biorad ChemiDoc MP imaging system. Blots were analysed using Image Lab software (Bio-rad laboratories).
Hrp linked secondary anti mouse or anti rabbit antibodies
Manufactured by Thermo Fisher Scientific
Sourced in United States
HRP-linked secondary anti-mouse or anti-rabbit antibodies are laboratory reagents used to detect the presence of mouse or rabbit primary antibodies in various immunoassay techniques, such as Western blotting, ELISA, and immunohistochemistry. These secondary antibodies are conjugated with horseradish peroxidase (HRP), an enzyme that can catalyze a colorimetric or chemiluminescent reaction, allowing for the visualization and quantification of the target protein or antigen.
Automatically generated - may contain errors
Lab products found in correlation
P stat1, by Cell Signaling Technology (2 mentions)
β actin, by Merck Group (2 mentions)
Image lab software, by Bio-Rad (2 mentions)
Chemidoc mp imaging system, by Bio-Rad (2 mentions)
P stat2, by Cell Signaling Technology (1 mentions)
Stat2, by Cell Signaling Technology (1 mentions)
P stat3, by Cell Signaling Technology (1 mentions)
Stat1, by Cell Signaling Technology (1 mentions)
Stat3, by Cell Signaling Technology (1 mentions)
2 protocols using hrp linked secondary anti mouse or anti rabbit antibodies
1
Protein Expression Analysis by Immunoblotting
2
Immunoblotting Analysis of STAT Proteins
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Cells were lysed in RIPA buffer (20 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA pH 8, 1% TRITON-X, and 0.5% SDS) supplemented with 1 mM PMSF, 1 mM Na3VO4, 5 μg/mL leupeptin, and 1 mM DTT and analysed by immunoblotting using antibodies against HA, pSTAT1, STAT1, pSTAT2, STAT2, pSTAT3, STAT3 (Cell Signalling Technology, Danvers, MA, USA) and β-actin (Sigma) and HRP-linked secondary anti-mouse or anti-rabbit antibodies (Invitrogen) and visualised using the Bio-rad ChemiDoc MP imaging system. Blots were analysed using Image Lab software (Bio-rad laboratories, New York, NY, USA).
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