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Bio plex bead based cytokine assay

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The Bio-Plex bead-based cytokine assay is a multiplex system that allows for the simultaneous detection and quantification of multiple cytokines, chemokines, and other analytes in a single sample. The assay utilizes color-coded magnetic beads coated with specific capture antibodies to capture and detect target analytes.

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Lab products found in correlation

Dnase 1, by Merck Group (2 mentions) Collagenase a, by Roche (2 mentions)

Spelling variants of this product

Bio-Plex (286 citations) Bio-Beads (148 citations) Bio-Plex assay (73 citations)

3 protocols using bio plex bead based cytokine assay

1

Cytokine Profile in Cell Culture

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Approximately 400 μL of culture medium was collected at the time of harvest. A panel of pro-inflammatory cytokines, including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6, IL-8 and macrophage chemotactic peptide-1 (MCP-1) was assessed. Levels of each were determined using the Bio-Plex bead-based cytokine assay from Bio-Rad Laboratories (Hercules, CA, United States).
Aslam M.N., McClintock S.D., Attili D., Pandya S., Rehman H., Nadeem D.M., Jawad-Makki M.A., Rizvi A.H., Berner M.M., Dame M.K., Turgeon D.K, & Varani J. (2020). Ulcerative Colitis-Derived Colonoid Culture: A Multi-Mineral-Approach to Improve Barrier Protein Expression. Frontiers in Cell and Developmental Biology, 8, 577221.
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2

Cytokine Profiling of Lymph Node Cells

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LDLN were digested via 1 mg/mL collagenase A (Roche) and 25 U/mL DNase I (Sigma Aldrich) in RPMI with 10% FCS for 45–60 minutes at 37°C and further dispersed via 18-gauge needle (10 mL syringe). RBCs were lysed and samples filtered through 100-micron nylon mesh. Single-cell suspensions of lymph nodes at a concentration of 2.5 × 106 cells/mL (0.2 mL plated per well) in a 96 well plate were re-stimulated with RSV 5 × 105 PFU or CRA 300 PNU as appropriate. The supernatants were collected at 48 hours and analyzed for the following cytokines: IFN-γ, IL-4, IL-5, and IL-13 using a Bio-Plex bead-based cytokine assay (Bio-Rad Laboratories, Hercules, CA).
Schuler CF I.V., Malinczak C.A., Best S.K., Morris S.B., Rasky A.J., Ptaschinski C., Lukacs N.W, & Fonseca W. (2020). Inhibition of uric acid or IL-1β ameliorates respiratory syncytial virus immunopathology and development of asthma. Allergy, 75(9), 2279-2293.
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3

Cytokine Production in Lung-Draining Lymph Nodes

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LDLN were digested via 1 mg/mL collagenase A (Roche) and 20 U/mL DNase I (Sigma‐Aldrich) in RPMI with 10% FCS for and further dispersed via a 18‐gauge needle (10 mL syringe). Red blood cells were lysed, and samples filtered through 100‐micron nylon mesh. Single‐cell suspensions of lung‐draining lymph nodes (500,000 cells per well) were restimulated with 300 PNU of CRA. The supernatants were collected at 48 h and analyzed for the following cytokines: IL‐4, IL‐5, IL‐13, and IFN‐γ using a Bio‐Plex bead‐based cytokine assay (Bio‐Rad Laboratories).
Asai N., Ethridge A.D., Fonseca W., Yagi K., Rasky A.J., Morris S.B., Falkowski N.R., Huang Y.J., Huffnagle G.B, & Lukacs N.W. (2023). A steroid‐resistant cockroach allergen model is associated with lung and cecal microbiome changes. Physiological Reports, 11(13), e15761.
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