Sera were collected from all mice on days 7, 14 and 24 and stored at −80 °C for further analysis. Serum samples were used as primary antibodies to coat the wells of microtitre plates overnight at 4 °C. Secondary antibodies included HRP-conjugated goat anti-mouse IgG, anti-mouse IgG1 and anti-mouse IgG2a (Santa Cruz, CA, USA). The antibody responses were monitored at OD450.
Anti mouse igg2a
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-mouse IgG2a is a secondary antibody that binds to the IgG2a subclass of mouse immunoglobulins. It is commonly used in various immunoassays and research applications to detect and quantify the presence of mouse IgG2a in biological samples.
Automatically generated - may contain errors
2 protocols using anti mouse igg2a
1
Serum Antibody Profiling in Mice
2
ELISA for M2e-FP Antibody Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ELISA was used to analyze influenza virus M2e-FP-specific antibody responses in the immunized mouse sera as previously described [27] , [30] (link) with some modifications. Briefly, ELISA plates were coated with recombinant M2e-FP proteins overnight at 4 °C, and blocked with 3% BSA-PBS for 2 h at 37 °C. Mouse sera at 2-serial dilutions were added to the plates, and incubated for 1 h at 37 °C. After 4 washes, the plates were incubated with HRP-conjugated anti-mouse IgG, anti-mouse IgG1, and anti-mouse IgG2a (1:5,000, Santa Cruz), respectively, for 1 h at 37 °C. The reaction was detected by 3,3′,5,5′-tetramethylbenzidine (TMB) substrate (Invitrogen), and stopped by 1 N H2SO4. The absorbance at 450 nm was measured using ELISA plate reader, and the related antibody titers were calculated.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!