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Quantitative colorimetric apoptosis kit

Manufactured by R&D Systems
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Sourced in United States

The Quantitative Colorimetric Apoptosis Kit is a laboratory tool designed to detect and quantify apoptosis, a type of programmed cell death, in biological samples. It utilizes a colorimetric detection method to provide a quantitative assessment of apoptosis levels.

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Lab products found in correlation

Synergy lx, by Agilent Technologies (2 mentions)

2 protocols using quantitative colorimetric apoptosis kit

1

Quantitative Colorimetric TUNEL Assay for PFOA-Induced Apoptosis

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We performed TUNEL assay for the identification of apoptotic cells following PFOA treatment using the quantitative colorimetric apoptosis kit according to the manufacturer’s instructions (Cat No. 4822–96-K-049, R&D Systems, Minneapolis, MN, USA). Briefly, ALC cells were treated with PFOA (500 μM) for 24 h and fixed with buffered formalin and methanol. Samples were treated with proteinase K solution, quenched by hydrogen peroxide, and labeled with terminal deoxynucleotidyl transferase (TdT). Cells that had incorporated labeled nucleotides were identified by horseradish peroxidase (HRP) and TACS-sapphire reagent. OD was measured at 450 nm using a microplate reader (SYNERGY LX, BioTek).
Fujiwara N., Yamashita S., Okamoto M., Cooley M.A., Ozaki K., Everett E.T, & Suzuki M. (2023). Perfluorooctanoic acid-induced cell death via the dual roles of ROS-MAPK/ERK signaling in ameloblast-lineage cells. Ecotoxicology and environmental safety, 260, 115089.
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2

Quantitative Colorimetric TUNEL Assay for PFOA-Induced Apoptosis

Check if the same lab product or an alternative is used in the 5 most similar protocols
We performed TUNEL assay for the identification of apoptotic cells following PFOA treatment using the quantitative colorimetric apoptosis kit according to the manufacturer’s instructions (Cat No. 4822–96-K-049, R&D Systems, Minneapolis, MN, USA). Briefly, ALC cells were treated with PFOA (500 μM) for 24 h and fixed with buffered formalin and methanol. Samples were treated with proteinase K solution, quenched by hydrogen peroxide, and labeled with terminal deoxynucleotidyl transferase (TdT). Cells that had incorporated labeled nucleotides were identified by horseradish peroxidase (HRP) and TACS-sapphire reagent. OD was measured at 450 nm using a microplate reader (SYNERGY LX, BioTek).
Fujiwara N., Yamashita S., Okamoto M., Cooley M.A., Ozaki K., Everett E.T, & Suzuki M. (2023). Perfluorooctanoic acid-induced cell death via the dual roles of ROS-MAPK/ERK signaling in ameloblast-lineage cells. Ecotoxicology and environmental safety, 260, 115089.
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