Anti traf6 8028s

Anti-Flag (F3165) and anti-HA (H6908) antibodies were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-Myc (sc40), anti-GAPDH (sc47724), anti-BECN1 (sc48341), and anti-Ub (sc-8017) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-TRAF6 (8028S) and anti-LC3 (2775S) were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-Mouse IgG H&L (HRP) (ab6728) was purchased from Abcam (Cambridge, MA, USA). Goat anti-Rabbit IgG antibody (HRP) (GTX213110-01) was purchased from GeneTex Inc. (Irvine, CA, USA). Lipopolysaccharide (LPS; L3024), dimethyl sulfoxide (DMSO; 472301), paraformaldehyde (P6148), Triton X-100 (T8787), 3-methyladenine (3-MA; M9281), and Dulbecco’s phosphate-buffered saline (DPBS; D8537) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Polyinosinic-polycytidylic acid (Poly(I:C), tlrl-pic) was purchased from InvivoGen (San Diego, CA, USA). Anti-β-arrestin 2 antibody (PA1-732) and Lipofectamine 2000 (11668019) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).

The reagents used in this study and their sources were Proteasome inhibitor PS341 (Bortezomib) and MG132 were purchased from MedChem Express (Princeton, NJ), and all of them were dissolved in dimethyl sulfoxide (DMSO) and stored at −80 °C. The antibodies purchased were Anti-TRAF2 (4724S), anti-TRAF6 (8028S), anti-cleaved Caspase-3 (9664S), anti-IKKβ (8943S), anti-IκBα (7543S), anti-phospho-IκBα (2859P), anti-NF-κB/p65 (8242S), anti-β-Actin (3700S), anti-Histone 3 (4499S), anti-K48-linkage-specific polyubiquitin (12805S), anti-GAPDH (5174S), anti-Rabbit IgG (2729), anti-mouse IgG (7076) (Cell Signaling Technology, Beverly, MA), anti-Parkin (ab15494, ab77924), and anti-phospho-IKKβ (ab59195) (Abcam, Cambridge, MA). The MTS assay (Cell Titer 96 Aqueous One Solution reagent) was purchased from Promega Corporation (Promega, Madison, WI). The CCK-8 assay kit (Cell Counting Kit-8, Dojindo, Japan), PI, and Annexin V-FITC Kits were purchased from Keygen Company (Nanjing, China). Dynabeads antibody-coupling kit was purchased from Life Technologies (Waltham, MA).