Rat insulin elisa assay kit

Serum glucose levels were measured using a Glucose oxidase commercial kit (Sigma, St. Louis, Mo, USA). Considering the variability of serum glucose levels in the rat, glycosylated haemoglobin (HbA1c) levels were used as an index of glucose control, through the DCA 2000+ latex immunoagglutination method (Bayer Diagnostics, Barcelona, Spain). Plasma insulin levels were quantified by using a rat insulin Elisa assay kit from Mercodia (Uppsala, Sweden). The steady state beta cell function of individual animals was evaluated using the previously validated homeostasis model assessment (HOMA) of β-cell function [31 (link)]. The formula used was as follows: [HOMA-β%] = 360 × fasting serum insulin (mU/L)/ fasting serum glucose (mg/dl) − 63. The values used (insulin and glucose) were obtained after an overnight of food deprivation. Serum triglycerides (TGs) were analysed on a Hitachi 717 analyser (Roche Diagnostics) using standard laboratorial methods. TGs kit was obtained from bioMérieux® (Lyon, France).

Puerarin (99.3% purity) was purchased from Chengdu Longquan High-Tech Natural Pharmaceutical Co., Ltd. (Chengdu, China). Methyl p-hydroxybenzoate (IS) was obtained from Meloneparma Biotechnology Co., Ltd. (Dalian, China). Heparin sodium and streptozotocin (STZ) were purchased from Sigma Chemical Co., Ltd. (St Louis, MO, USA). The glucose test kit was purchased from Roche Co., Ltd. (Shanghai, China). Triglyceride and total cholesterol kits were from Beijing BHKT Clinical Reagent Co., Ltd Beijing, China. Rat insulin ELISA assay kits were supplied by Mercodia Co., Ltd. (Uppsala, Sweden). The GoScript reverse transcriptase kit and GoTaq^® qPCR Master Mix kit were purchased from Promega Co., Ltd. (Beijing, China). TRIzol was purchased from Invitrogen Co., Ltd. (Shanghai, China). UGT primers were synthesized by Beijing Dingguo Changsheng Biotechnology Co., Ltd. (Beijing, China). Anti-Ugt1a1 (abs-134323) and Anti-Ugt1a7 (sc-377075) were purchased from Absin Bioscience Inc. (Shanghai, China) and Santa Cruz Co., Ltd. (Shanghai, China), respectively. Methanol and acetonitrile (HPLC grade) were purchased from Concord Technology Co., Ltd. (Tianjin, China). All other reagents were analytical grade and were commercially available.