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Anti ap2γ

Manufactured by Santa Cruz Biotechnology
Sourced in Japan

Anti-AP2γ is a laboratory reagent used for the detection and analysis of the AP2γ protein in biological samples. It functions as a specific antibody that binds to the AP2γ protein, enabling its identification and quantification in various research applications.

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2 protocols using anti ap2γ

1

Embryonic Chromatin Modification Profiling

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Embryos were dissected from the decidua and Reichert’s membrane and treated as previously described (Nichols et al., 2009 (link)). Primary antibodies used are as follows: anti-H3K9me2 (Millipore, CA, 17–648), anti-H3K9me2 (Abcam, UK, ab1220), anti-GFP (Nacalai tesque, Japan, GF090R), anti-G9a (R&D Systems, MN, A8620A), anti-GLP (R&D Systems, MN, PP-B0422-00), anti-cleaved Caspase 3 (Abcam, UK, ab32042), anti-Ki67 (BD Bioscience, NJ, 550609), anti-NANOG (Cosmobio, Japan, REC-RCAB002P-F), anti-AP2γ (Santa Cruz, CA, sc-9877). All imaging was performed using SP5 or SP7 confocal microscope (Leica, Germany).
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2

Immunohistochemical Analysis of Mammary Gland

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Immunohistochemical analysis was accomplished on formalin-fixed, paraffin-embedded samples from the fourth mammary gland. The following antibodies were used for analysis: anti-AP-2γ, 1:100 (Santa Cruz Biotechnology, no. sc-12762); anti-CRE, 1:50 (Cell Signaling Technology, no. 15036); anti-CK5, 1:100 (Abcam, ab5235); and anti-CK8/18, 1:500 (Abcam, ab53280). Standard protocols were followed, and Vector ABC kits used for amplification. Bright-field imaging was collected on an Olympus BX-51 microscope.
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