Normal human dermal fibroblasts (NHDF) and HEK293T cells were obtained from ATCC and cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (HyClone), 100 units/ml penicillin, 100ug/mL streptomycin and 100ug/mL glutamine (ThermoFisher). Human aortic endothelial cells (hAEC) (CC-2535; Lonza) were cultured in EBM-2 basal medium with EGM-2 SingleQuots supplement excluding Heparin (Lonza), as well as 10% FBS, penicillin, and streptomycin. Kasumi-3 cells were obtained from ATCC and maintained in RPMI 1640 with 20% FBS and penicillin, streptomycin, and glutamine. M2-10B4 and S1/S1 stromal cells were obtained from Stem Cell Technologies and maintained in DMEM with 10% fetal bovine serum and penicillin, streptomycin, and glutamine. All cells were maintained at 37°C and 5% CO2.
Kasumi 3 cells
Manufactured by American Type Culture Collection
The Kasumi-3 cell line is a human acute myeloid leukemia (AML) cell line. It is a suspension culture cell line derived from the peripheral blood of a 53-year-old male patient with AML.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (3 mentions)
Streptomycin, by Thermo Fisher Scientific (3 mentions)
Glutamine, by American Type Culture Collection (1 mentions)
Glutamine, by STEMCELL (1 mentions)
Streptomycin, by STEMCELL (1 mentions)
Penicillin, by STEMCELL (1 mentions)
Glutamine, by Thermo Fisher Scientific (1 mentions)
Penicillin, by American Type Culture Collection (1 mentions)
Streptomycin, by American Type Culture Collection (1 mentions)
M2 10b4 and s1 s1 stromal cells, by STEMCELL (1 mentions)
Egm 2 singlequots supplement excluding heparin, by Lonza (1 mentions)
Hek293t, by American Type Culture Collection (1 mentions)
Penicillin, by Lonza (1 mentions)
Streptomycin, by Lonza (1 mentions)
Rpmi 1640, by American Type Culture Collection (1 mentions)
Glutamax 1, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Gentamicin, by Merck Group (1 mentions)
2 protocols using kasumi 3 cells
1
Culturing Various Human Cell Lines
2
Cell Culture Conditions for HFFs and Kasumi-3
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HFFs (SCRC-1041; American Tissue Culture Collection [ATCC]) were grown in Dulbecco’s modified Eagle’s medium (DMEM; Hyclone) with 10% fetal bovine serum (FBS; HyClone), GlutaMAX-I (Gibco), and penicillin (100 U/mL)-streptomycin (100 μg/mL) (Gibco). Kasumi-3 cells (CRL-2725; ATCC) were cultured in Roswell Park Memorial Institute medium (RPMI 1640; ATCC) supplemented with 20% FBS, penicillin–streptomycin, and 100 μg/mL gentamicin (Sigma). Cells were incubated at 37 °C in a 5% CO2 atmosphere.
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