Pack ods am 303 column

HPLC measurements were performed according to a previous report using a JASCO HPLC system (Jasco Corp., Tokyo, Japan) [10 (link)]. A YMC-PACK ODS AM 303 column (5 µm, 250 mm × 4.6 mm, YMC Co., Kyoto, Japan) was used as the stationary phase and was maintained at 40 °C. A linear gradient elution system was employed with a mobile phase comprised two solvents, A (0.05 M sodium dihydrogen phosphate) and B (0.05 M sodium dihydrogen phosphate, and acetonitrile (30:70, v/v)), programmed for PP, PB, and PV as follows: 0–7 min (30–100% B), 7–10 min (100% B), 10–15 min (100–30% B). For PC and PH, the elution program used was 0–7 min (50–100% B), 7–10 min (100% B), and 10–15 min (100–50% B) at a constant flow rate of 1 mL/min. A detection wavelength of 210 nm was used and monitored for 15 min for each sample, with retention times of 7.4, 8.9, 9.6, 9.4, and 10.7 min for PP, PB, PV, PC, and PH, respectively.

HPLC measurements were carried out according to a previous report using a JASCO HPLC system (Jasco Corp., Tokyo, Japan) [32 (link)]. The stationary phase was a YMC-PACK ODS AM 303 column (5 µm, 250 mm × 4.6 mm, YMC Co., Kyoto, Japan) maintained at 40 ± 0.5 °C. The mobile phase comprised of two solvents, A (0.05 M sodium dihydrogen phosphate) and B (0.05 M sodium dihydrogen phosphate and acetonitrile (30:70, v/v)), programmed as follows: 0–7 min (30–100% B), 7–10 min (100% B), and 10–15 min (100–30% B) at a flow rate of 1 mL/min. A detection wavelength of 210 nm was used and each sample was monitored for 15 min. The retention time for PB was 8.9 min.