SW1990 cells were first cultured under normal culture conditions. Cell proliferation was analyzed using the MTS Cell Proliferation Kit (ab197010, Abcam). The cells were treated with 10 μM gemcitabine (GEM; LY-188011, MedChemExpress) for 5 days to detect the sensitivity of SW1990 cells with DMSO as a NC.
Mts cell proliferation kit
Manufactured by Abcam
The MTS Cell Proliferation Kit is a colorimetric assay used to quantify cell proliferation and viability. It measures the metabolic activity of cells, which is directly proportional to the number of living cells in culture.
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2 protocols using mts cell proliferation kit
1
Evaluating Gemcitabine Sensitivity in SW1990 Cells
2
MTS-Based Cell Proliferation Assay
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The MTS Cell Proliferation Kit (Abcam) was used to measure the cell proliferation rate at six different time points after seeding (0, 1, 2, 7, 14, and 21 days). In a final volume of 200 μl of cell culture medium, 20 μl of MTS was added and incubated for 4 h at 37°C in standard culture conditions. After incubation, the optical density was measured at 490–500 nm by a Glomax Multi+ Detection System (Promega).
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