The largest database of trusted experimental protocols

Anti ve cadherin igg

Manufactured by Abcam
Sourced in Ireland, United Kingdom

Anti-VE-Cadherin IgG is a laboratory reagent used in research applications. It is an antibody that specifically binds to the VE-Cadherin protein.

Automatically generated - may contain errors

2 protocols using anti ve cadherin igg

1

Molecular Mechanisms of Endothelial Barrier Regulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Unless otherwise stated, all reagents were purchased from Sigma-Aldrich (Dublin, Ireland). Western blotting primary antisera were purchased from the following sources: anti-occludin IgG, anti-claudin-5 IgG, and anti-ZO-1 IgG (Thermo Fischer Scientific, Dublin, Ireland); anti-VE-Cadherin IgG (Abcam, Cambridge, UK); anti-β-actin (Cell Signaling Technologies Inc., Danvers, MA, USA). Horseradish peroxidase (HRP)-conjugated secondary antisera for all targets were purchased from Cell Signaling Technologies Inc. COMP-Ang1 was purchased from Enzo Life Sciences (Exeter, UK). For Tie2 experiments, dual Ambion Silencer Select small interfering RNA (siRNA) constructs (assay IDs 13983 and s13984 against TEK/Tie2) were purchased from Thermo Fisher Scientific. An anti-Tie2 IgG and associated donkey anti-goat secondary IgG were purchased from R&D Systems (Abingdon, UK).
+ Open protocol
+ Expand
2

Endothelial Barrier Regulation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Unless otherwise stated, all reagents were purchased from Sigma-Aldrich (Dublin, IRL). Cytokines (TNF-α, IL-6), apocynin and NSC23766 were purchased from Millipore (Cork, IRL). Primary antisera were purchased from the following sources: Anti-occludin IgG, anti-claudin-5 IgG, and anti-ZO-1 IgG (Bio-Sciences, Dublin, IRL); Anti-VE-cadherin IgG (Abcam, Cambridge, UK); Anti-gp91 IgG, anti-p47 IgG, and anti-GAPDH IgG (Santa Cruz Biotechnology, CA, USA); HRP-conjugated secondary antisera for VE-cadherin, occludin, claudin-5, and GAPDH were purchased from Cell Signalling Technologies Inc. (MA, USA). HRP-conjugated secondary antisera for gp91 and p47 were purchased from Sigma Aldrich. siRNA constructs for gp91 (SC35503, RefSeq NM_000397.3) and p47 (SC29422, RefSeq NM_000265.5) were obtained from Santa Cruz Biotechnology.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!