Unless otherwise stated, all reagents were purchased from Sigma-Aldrich (Dublin, Ireland). Western blotting primary antisera were purchased from the following sources: anti-occludin IgG, anti-claudin-5 IgG, and anti-ZO-1 IgG (Thermo Fischer Scientific, Dublin, Ireland); anti-VE-Cadherin IgG (Abcam, Cambridge, UK); anti-β-actin (Cell Signaling Technologies Inc., Danvers, MA, USA). Horseradish peroxidase (HRP)-conjugated secondary antisera for all targets were purchased from Cell Signaling Technologies Inc. COMP-Ang1 was purchased from Enzo Life Sciences (Exeter, UK). For Tie2 experiments, dual Ambion Silencer Select small interfering RNA (siRNA) constructs (assay IDs 13983 and s13984 against TEK/Tie2) were purchased from Thermo Fisher Scientific. An anti-Tie2 IgG and associated donkey anti-goat secondary IgG were purchased from R&D Systems (Abingdon, UK).
Anti ve cadherin igg
Manufactured by Abcam
Sourced in Ireland, United Kingdom
Anti-VE-Cadherin IgG is a laboratory reagent used in research applications. It is an antibody that specifically binds to the VE-Cadherin protein.
Automatically generated - may contain errors
2 protocols using anti ve cadherin igg
1
Molecular Mechanisms of Endothelial Barrier Regulation
2
Endothelial Barrier Regulation Assay
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Unless otherwise stated, all reagents were purchased from Sigma-Aldrich (Dublin, IRL). Cytokines (TNF-α, IL-6), apocynin and NSC23766 were purchased from Millipore (Cork, IRL). Primary antisera were purchased from the following sources: Anti-occludin IgG, anti-claudin-5 IgG, and anti-ZO-1 IgG (Bio-Sciences, Dublin, IRL); Anti-VE-cadherin IgG (Abcam, Cambridge, UK); Anti-gp91 IgG, anti-p47 IgG, and anti-GAPDH IgG (Santa Cruz Biotechnology, CA, USA); HRP-conjugated secondary antisera for VE-cadherin, occludin, claudin-5, and GAPDH were purchased from Cell Signalling Technologies Inc. (MA, USA). HRP-conjugated secondary antisera for gp91 and p47 were purchased from Sigma Aldrich. siRNA constructs for gp91 (SC35503, RefSeq NM_000397.3) and p47 (SC29422, RefSeq NM_000265.5) were obtained from Santa Cruz Biotechnology.
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