Mouse anti rabbit glyceraldehyde 3 phosphate dehydrogenase gapdh antibody

Astaxanthin was provided by Fuji Chemical Industries Co., Ltd. (Toyama, Japan). TRI reagent was purchased from Sigma-Aldrich Corp. (St. Louis, MO, USA). A high-capacity cDNA synthesis kit was purchased from Applied Biosystems (Foster City, CA, USA). RIPA (radioimmunoprecipitation assay) buffer and a protease inhibitor cocktail were purchased from Nacalai Tesque, Inc. (Kyoto, Japan). A rabbit anti-rat AQP3 antibody was purchased from Alomone Labs (Jerusalem, Israel). A mouse anti-rabbit glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody was purchased from Merck Millipore (Darmstadt, Germany). A donkey anti-rabbit IgG-HRP (horseradish peroxidase) antibody, sheep anti-mouse IgG-HRP antibody, and enhanced chemiluminescence (ECL) Prime Western blotting detection reagents were purchased from GE Healthcare (Chicago, IL, USA). A glycerol colorimetric assay kit was purchased from Cayman Chemical (Ann Arbor, MI, USA).

Erlotinib was purchased from LC Laboratories (Woburn, MA, USA). Gefitinib was purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). TRI reagent was purchased from Sigma-Aldrich Corp. (St. Louis, MO, USA). A high-capacity cDNA synthesis kit was purchased from Applied Biosystems (Foster City, CA, USA). The rabbit anti-rat aquaporin-3 (AQP3) antibody was purchased from Alomone Labs (Jerusalem, Israel). The mouse anti-rabbit glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody and sheep anti-mouse IgG-HRP antibody were purchased from Merck Millipore (Darmstadt, Germany). The donkey anti-rabbit IgG-HRP antibody was purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). The rabbit anti-human EGFR antibody, rabbit anti-human phospho (p)-EGFR antibody, rabbit anti-human p44/42 mitogen-activated protein kinase (MAPK) antibody, and rabbit anti-human p-p44/42 MAPK antibody were purchased from Cell Signaling Technology (Beverly, MA, USA). Alexa Fluor 488 donkey anti-rabbit IgG was purchased from Thermo Fisher Scientific (Waltham, MA, USA). An enhanced chemiluminescence (ECL) prime detection reagent was purchased from GE Healthcare (Chicago, IL, USA). The cell proliferation reagent water-soluble tetrazolium salt (WST-1) was purchased from Roche (Mannheim, Germany).