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Eclipse ti inverted fluorescent microscope

Manufactured by Hamamatsu Photonics
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The Eclipse Ti Inverted Fluorescent Microscope is a high-performance optical microscope designed for fluorescence imaging applications. It features an inverted optical configuration, allowing for the observation of samples from the bottom. The microscope is equipped with advanced fluorescence capabilities, enabling the visualization and analysis of fluorescently labeled specimens.

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Lab products found in correlation

Digital camera c11440 orca flash 2, by Hamamatsu Photonics (2 mentions) Alexa fluor 647 anti mouse ly 6g ly 6c gr 1, by BioLegend (2 mentions) Prolong gold antifade reagent, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Eclipse Ti inverted microscope Eclipse Ti microscope Ti inverted microscope Eclipse Ti Ti microscope Inverted microscope

2 protocols using eclipse ti inverted fluorescent microscope

1

Neutrophil Immunofluorescence Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
Once cryo-sections were obtained as described in the previous paragraph, immunofluorescence (IF) staining was performed as previously reported49 . Briefly, slides were thawed and blocked with BSA 5% (Sigma-Aldrich®) PBS 1x solution. After washing, they were incubated overnight at 4 degrees with anti-neutrophil antibody (Alexa Fluor 647 anti-mouse Ly-6G/Ly-6C (Gr-1) Biolegend®). Excess of the anti-neutrophil antibody was washed out with PBS 1X. Cells nuclei were stained with DAPI. Slides were sealed with ProLong Gold antifade reagent (Life technologies™). Images were captured with a Nikon® Eclipse Ti Inverted Fluorescent Microscope equipped with Hamamatsu Digital Camera C11440 ORCA-Flash 2.8.
Molinaro R., Corbo C., Martinez J.O., Taraballi F., Evangelopoulos M., Minardi S., Yazdi I.K., Zhao P., De Rosa E., Sherman M., De Vita A., Furman N.E., Wang X., Parodi A, & Tasciotti E. (2016). Biomimetic proteolipid vesicles for targeting inflamed tissues. Nature materials, 15(9), 1037-1046.
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2

Neutrophil Immunofluorescence Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
Once cryo-sections were obtained as described in the previous paragraph, immunofluorescence (IF) staining was performed as previously reported49 . Briefly, slides were thawed and blocked with BSA 5% (Sigma-Aldrich®) PBS 1x solution. After washing, they were incubated overnight at 4 degrees with anti-neutrophil antibody (Alexa Fluor 647 anti-mouse Ly-6G/Ly-6C (Gr-1) Biolegend®). Excess of the anti-neutrophil antibody was washed out with PBS 1X. Cells nuclei were stained with DAPI. Slides were sealed with ProLong Gold antifade reagent (Life technologies™). Images were captured with a Nikon® Eclipse Ti Inverted Fluorescent Microscope equipped with Hamamatsu Digital Camera C11440 ORCA-Flash 2.8.
Molinaro R., Corbo C., Martinez J.O., Taraballi F., Evangelopoulos M., Minardi S., Yazdi I.K., Zhao P., De Rosa E., Sherman M., De Vita A., Furman N.E., Wang X., Parodi A, & Tasciotti E. (2016). Biomimetic proteolipid vesicles for targeting inflamed tissues. Nature materials, 15(9), 1037-1046.
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