Cells were seeded on a 24-well the day before the experiment and then fixed with 4% PFA for 15 minutes at room temperature, permeabilized with 0.5% Triton X-100 in 1X PBS and incubated with blocking solution (0.3% Triton X-100, 5% FBS in 1X PBS) for 1 hour. Primary antibody incubation was carried out at 4°C overnight, followed by three washes with 1X PBS. Proper secondary antibodies were eventually used and images acquired using a confocal microscope (Leica TCS SP5). The following antibodies were employed: rat anti-Nestin (Santa Cruz Biotechnology), rabbit anti-Sox2 (GeneTex), goat Alexa Fluor 546 and 647 (Life Technologies). Nuclei were stained using Hoechst 33342 (Life Technologies), diluted 1:20000 in 1X PBS.
Rat anti nestin
Manufactured by Santa Cruz Biotechnology
Rat anti-Nestin is a primary antibody that recognizes the intermediate filament protein Nestin. Nestin is a well-established marker for neural stem and progenitor cells. This antibody can be used to detect and localize Nestin in various cell types and tissues.
Automatically generated - may contain errors
2 protocols using rat anti nestin
1
Immunofluorescence Staining of Neural Stem Cells
2
Immunofluorescence Staining of Neural Progenitors
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Cells were seeded on a 24-well the day before the experiment and then fixed with 4% PFA for 15 minutes at room temperature, permeabilized with 0.5% Triton X-100 in 1X PBS and incubated with blocking solution (0.3% Triton X-100, 5% FBS in 1X PBS) for 1 hour.
Primary antibody incubation was carried out at 4 °C overnight, followed by three washes with 1X PBS. Proper secondary antibodies were eventually used and images acquired using a confocal microscope (Leica TCS SP5). The following antibodies were used: rat anti-Nestin (Santa Cruz Biotechnology), rabbit anti-Sox2 (GeneTex), goat Alexa Fluor 546 and 647 (Life Technologies). Nuclei were stained using Hoechst 33342 (Life Technologies), diluted 1:20000 in 1X PBS.
Primary antibody incubation was carried out at 4 °C overnight, followed by three washes with 1X PBS. Proper secondary antibodies were eventually used and images acquired using a confocal microscope (Leica TCS SP5). The following antibodies were used: rat anti-Nestin (Santa Cruz Biotechnology), rabbit anti-Sox2 (GeneTex), goat Alexa Fluor 546 and 647 (Life Technologies). Nuclei were stained using Hoechst 33342 (Life Technologies), diluted 1:20000 in 1X PBS.
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