Masson trichrome staining kit

Manufactured by Abcam

Sourced in United Kingdom

The Masson trichrome staining kit is a set of reagents used to stain collagen fibers in histological samples. It provides a selective staining of connective tissues, allowing for the visualization of collagen fibrils in a blue-green color against a red background of other tissue components.

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Lab products found in correlation

Anti mmp 9, by Santa Cruz Biotechnology (1 mentions) Sirius red, by Merck Group (1 mentions) Hrp dab ihc kit, by Abcam (1 mentions) Evans blue, by Merck Group (1 mentions) Anti mmp 13, by Santa Cruz Biotechnology (1 mentions) Hematoxylin eosin, by Merck Group (1 mentions) Anti emmprin, by Abcam (1 mentions) Hrp conjugated secondary antibody, by Abcam (1 mentions) Anti cd68, by Abcam (1 mentions) Triton x 100, by Merck Group (1 mentions) Diazepam, by Roche (1 mentions) Ketamine, by Pfizer (1 mentions) Isoflurane, by Abbvie (1 mentions) Propofol, by Fresenius (1 mentions) Amiodarone, by Sanofi (1 mentions) Fentanyl, by Kern & Sohn (1 mentions)

3 protocols using masson trichrome staining kit

Measuring Mouse Cardiomyocyte Size and Fibrosis

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Mouse heart tissues were cryopreserved with an optimum cutting temperature compound (Tissue-Tek O.C.T. Compound; Sakura, Torrance, CA, USA) and sectioned into 6-μm-thick slices. To measure cell size, a hematoxylin/eosin (H&E) staining kit (9990001; Fisher Scientific, Hampton, NH, USA) was used to stain the sectioned hearts. The cells were stained pink, and the nuclei were blue. The cross-sectional area was imaged using light microscopy (E400; Nikon, Tokyo, Japan) and analyzed using ImageJ software (NIH) at ×40 magnification. A minimum of 150 cardiomyocytes were measured per heart. To measure the fibrotic areas, a Masson trichrome staining kit (ab150686; Abcam, Trumpington, Cambridge, UK) was used to stain the sectioned hearts. The fibrotic area was stained blue, and the normal tissue was stained red. Finally, the fibrotic area was calculated as the ratio of the total area of fibrosis to the total area of the section using ImageJ software.

Kepreotis S.V., Oh J.G., Park M., Yoo J., Lee C., Mercola M., Hajjar R.J, & Jeong D. (2024). Inhibition of miR-25 ameliorates cardiac and skeletal muscle dysfunction in aged mdx/utrn haploinsufficient (+/−) mice. Molecular Therapy. Nucleic Acids, 35(2), 102174.

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Masson Trichrome Staining of Liver Collagen

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Masson trichrome staining was used to assess the collagen fiber of the liver using a Masson Trichrome Staining Kit (Abcam, UK). Concisely, sections were deparaffinised, and preheated Bouin's fluid had been added for one hour convoyed by a 10-minute cooling period. The slides were washed in tap water till the section became completely clean. The slides were stained with working Weigert's iron haematoxylin for five minutes. Biebrich Scarlet-Acid Fuchsin Solution was applied to the slides for 15 minutes. The differentiation in phosphomolybdic/phosphotungstic acid solution was performed for 10-15 minutes. Without rinsing, aniline blue solution was applied to the slides for 5-10 minutes. Acetic acid solution (1%) was applied to the slides. Dehydration was performed very quickly in 2 changes of 95% ethyl alcohol, followed by 2 changes of absolute alcohol, and the slides were cleared via xylene and mounting was done.

Alsahli M.A., Almatroodi S.A., Almatroudi A., Khan A.A., Anwar S., Almutary A.G., Alrumaihi F, & Rahmani A.H. (2021). 6-Gingerol, a Major Ingredient of Ginger Attenuates Diethylnitrosamine-Induced Liver Injury in Rats through the Modulation of Oxidative Stress and Anti-Inflammatory Activity. Mediators of Inflammation, 2021, 6661937.

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Histochemistry and Immunohistochemistry Protocol

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Evans Blue, triphenyl tetrazolium chloride, hematoxylin-eosin, sirius red, Triton X-100, calcium chloride, zinc chloride, and acetic acid were from Merck (Madrid, Spain). The Masson trichrome staining kit, anti-EMMPRIN, anti-CD68, HRP/DAB IHC kit, and HRP-conjugated secondary antibodies were from Abcam (Cambridge, United Kingdom). Anti-MMP-9 and anti-MMP-13 were from Santa Cruz (Heidelberg, Germany). Ketamine was from Pfizer (New York, NY); the isoflurane was from Abbvie (North Chicago, IL); the propofol was from Fresenius (Bad Homburg, Germany); the fentanyl was from Kern Pharma (Madrid, Spain); the diazepam was from Roche (Basel, Switzerland); and the amiodarone was from Sanofi Aventis (Gentilly, France).

Ramirez-Carracedo R., Sanmartin M., Ten A., Hernandez I., Tesoro L., Diez-Mata J., Botana L., Ovejero-Paredes K., Filice M., Alberich-Bayarri A., Martí-Bonmatí L., Largo-Aramburu C., Saura M., Zamorano J.L, & Zaragoza C. (2022). Theranostic Contribution of Extracellular Matrix Metalloprotease Inducer-Paramagnetic Nanoparticles Against Acute Myocardial Infarction in a Pig Model of Coronary Ischemia-Reperfusion. Circulation. Cardiovascular Imaging, 15(6), e013379.

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