High performance liquid chromatography (HPLC) grade acetonitrile and methanol were purchased from Thermo Fisher Scientific Co., Ltd. (Fair Lawn, NJ, USA). Ultra-pure water was obtained from Hangzhou Wahaha Group Co., Ltd. (Hangzhou, China), N,O-bis(trimethylsilyl)trifluoro-acetamide (BSTFA) with 1% trimethylchlorosilane (TMCS), O-methoxyamine-HCl (MOX), succinic-d4 acid were purchased from Sigma–Aldrich (NJ, USA). Adenine, 4% tissue cell fixative, yeast powder, urease activity test kit, hematoxylin-eosin staining (HE) staining kit, Masson staining kit and standards for creatinine, uric acid, hypoxanthine, xanthine, urea, etc. were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China) and the purity of the above standands are all greater than 98%. The urea nitrogen test kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Other chromatographic reagents were obtained from domestic reagent companies.
Uric acid
Manufactured by Solarbio
Sourced in China
Uric acid is a chemical compound used in laboratory settings for the measurement and analysis of uric acid levels. It is a key component in various biochemical assays and diagnostic tests.
Automatically generated - may contain errors
Lab products found in correlation
Hplc grade acetonitrile, by Thermo Fisher Scientific (1 mentions)
Tissue cell fixative, by Solarbio (1 mentions)
Urea nitrogen test kit, by Nanjing Jiancheng (1 mentions)
Masson staining kit, by Solarbio (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Hematoxylin eosin staining he staining kit, by Solarbio (1 mentions)
Adenine, by Solarbio (1 mentions)
Yeast powder, by Solarbio (1 mentions)
Hypoxanthine, by Solarbio (1 mentions)
Xanthine, by Solarbio (1 mentions)
Hemoglobin, by Solarbio (1 mentions)
Mannitol, by Solarbio (1 mentions)
Sodium pyruvate, by Solarbio (1 mentions)
2 protocols using uric acid
1
HPLC-based Metabolite Extraction and Analysis
2
Quenching CAP-induced Cellular Stress
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Mannitol (200 mM, Catalog Number: M8140, Solarbio, China), sodium pyruvate (10 mM, Catalog Number: SP0100, Solarbio, China), uric acid (100 μM, Catalog Number: IU0150, Solarbio, China), tiron (20 mM, Catalog Number: T104954, Aladdin, China), hemoglobin (20 μM, Catalog Number: H8020, Solarbio, China), and monopotassium phosphate (1 mM, Catalog Number: P7392, Solarbio, China) were used to quench hydroxyl radical (OH·), hydrogen peroxide (H2O2), ozone (O3), superoxide anion (O2·-), nitric oxide (NO·), and electron (e-), respectively.
After treating cells with CAP for 2 or 4 min followed by incubation for 1 h, the quencher of each CAP component was added, separately. Viruses were added to cells and incubated for 1 h. The medium was refreshed, and cells were cocultured with viruses for additional 46 h prior to subsequent analyses.
After treating cells with CAP for 2 or 4 min followed by incubation for 1 h, the quencher of each CAP component was added, separately. Viruses were added to cells and incubated for 1 h. The medium was refreshed, and cells were cocultured with viruses for additional 46 h prior to subsequent analyses.
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