Proteasomal inhibitor (Mg132), Medroxyprogesterone 17-acetate (MPA), phosphatidylinositol 3-kinase (PI3K) inhibitor (Wortmannin) and mammalian target of Rapamycin (mTOR) inhibitor (Rapamycin) were obtained from Sigma-Aldrich (St. Louis, MO). Fetal bovine serum (FBS) and gentamycin sulfate were purchased from HyClone (South Logan, Utah). 8-Bromo-cAMP was obtained from Enzo Life Sciences Inc (Farmingdale, NY). Akt 1 (2938), Akt 2 (2964), AKT total (9272), phospho-AKT (4060), phospho-IκBα (2859), IκBα (9242), phospho-mTOR (2974), mTOR (2983), Par-4 (2328), PARP (9532), FoxO1 (2880), Slug (9585) and Ubiquitin (3933) primary antibodies were purchased from Cell Signaling (Danvers, MA). Anti-Akt 3 (07–383) was obtained from Millipore (Billerica, MA), beta-tubulin antibody and HRP-conjugated GAPDH(ab9484) antibodies were from Abcam (Cambridge, MA) and monoclonal anti-β-actin-peroxidase from Sigma-Aldrich (St. Louis, MO). Horseradish peroxidase (HRP)-conjugated anti-rabbit and anti-mouse secondary antibodies were provided by Bio-Rad Laboratories (Mississauga, ON, Canada). A full description of antibodies, catalog numbers and concentration can be found in S1 Table .
Ubiquitin 3933
Manufactured by Cell Signaling Technology
Sourced in United States
Ubiquitin (3933) is a recombinant protein produced in E. coli. It is a small, highly conserved protein that plays a crucial role in the regulation of various cellular processes by targeting proteins for degradation or modifying their function.
Automatically generated - may contain errors
Lab products found in correlation
Pi3k inhibitor wortmannin, by Merck Group (1 mentions)
8 bromo camp, by Enzo Life Sciences (1 mentions)
Monoclonal anti β actin peroxidase, by Merck Group (1 mentions)
Gapdh ab9484, by Abcam (1 mentions)
Irdye 800 cw goat anti rabbit igg 926 32211, by LI COR (1 mentions)
Phosphor stat3 9145, by Cell Signaling Technology (1 mentions)
Odyssey system, by LI COR (1 mentions)
2 protocols using ubiquitin 3933
1
Signaling Pathway Inhibitor Assays
2
Ubiquitin and Phospho-Stat3 in GEMM KPC Mice
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Lysates made from quadriceps skeletal muscles collected from GEMM KPC mice and the sex‐matched and age‐matched genotype control mice at early and late tumour stages were subjected to western blotting. The primary antibodies used were ubiquitin (3933) and phosphor‐Stat3 (9145) from Cell Signalling Technology, Danvers, MA, USA, and GAPDH (ab9484) from Abcam, Cambridge, MA, USA. The secondary antibodies used were IRDye 800CW goat anti‐rabbit IgG (926‐32211) and IRDye 680LT goat anti‐mouse IgG2b‐specific (926‐68052) from LICOR Biosciences, Lincoln, NE, USA. Signal was detected using a LI‐COR Odyssey system.
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