To explore the pharmacokinetic properties of YCHD following intragastric administration in rats, a rapid and sensitive method involving the use of UHPLC-MS/MS (Agilent 6460 series, Agilent Technologies, Santa Clara, CA, United States) was developed and validated for the simultaneous quantification of 10 active components of rat plasma. The quantification was conducted in ESI negative ionization mode, and the mass spectrometry conditions were as follows: capillary voltage, 3500 V; gas flow, 12 L/min; nebulizer, 40 psi; gas temperature, 350°C; and delta EMV (-), 400. A 5 μL extraction sample was injected into the column (Agilent SB-C18 column, 2.1 mm × 50 mm, 1.8 μm) and eluted at 0.4 mL/min with a gradient elution of water (with 0.4% v/v formic acid) (A) and acetonitrile (B) (0–3 min, 5–60% B; 3–5 min, 60–90% B; 5–5.1 min, 90–5% B; and re-equilibration for 3 min).
Agilent 6460 series
Manufactured by Agilent Technologies
Sourced in United States
The Agilent 6460 series is a line of high-performance triple quadrupole mass spectrometers designed for a wide range of analytical applications. These instruments provide precise and accurate mass analysis capabilities for qualitative and quantitative analysis.
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Lab products found in correlation
2 protocols using agilent 6460 series
1
Pharmacokinetic Analysis of YCHD in Rats
2
UPLC-MS/MS Metabolite Profiling Protocol
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The prepared samples were analyzed by an UPLC system (Agilent 1290 series, USA) couple with a triple quadrupole mass spectrometer system (Agilent 6460 series, USA). In brief, 3 μL of each sample was injected into an UPLC BEH Amide column (1.7 μm, 2.1×100 mm, Waters) at 25ºC. The mobile phase composed of water including 25 mM CH3COONH4 and 25 mM NH4OH (solvent A) and 100% ACN (solvent B). The gradient for metabolites elution solvent B switched from 95% to 65% after 14 min, then declined to 40% for 2 min and kept for 2 min, finally backed to 95% in 0.1 min and maintained for 4.9 min. The flow rate was 0.3 mL/min. MS conditions were set as followings: scanning method, MRM. ESI source temperature, 100ºC. Desolvation temperature, 300ºC. Desolvation gas flow, 3.0 L/min.
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