Biopsies were fixed in 10% neutral buffered formalin, embedded in paraffin, and stained with hematoxylin–eosin. Immunohistochemical staining and in situ hybridization were performed on an automated stainer (Bond Max; Leica Biosystems). The presence of EBV was demonstrated by in situ hybridization with a probe for small RNA-encoding regions 1 and 2 (EBER) probe (Dako). Antibodies used were: anti-CD3 (polyclonal rabbit, CD3ε, A0452; Dako Agilent), anti-CD79a (monoclonal mouse IgG1k, JCB117; Dako), anti-caldesmon (monoclonal mouse IgG, clone h-CD [M3557]; Dako), anti-PAX5 (clone DAK-PAX5; 1/30; Dako), and anti-4-1BBL/CD137L (polyclonal rabbit IgG, ab64912; Abcam).
Clone dak pax5
Manufactured by Agilent Technologies
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The Clone DAK-PAX5 is a laboratory equipment product designed for research purposes. It serves as a tool for analysis and investigation, but its specific intended use should not be extrapolated beyond its core function.
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Lab products found in correlation
2 protocols using clone dak pax5
1
Immunohistochemical analysis of EBV
2
Immunohistochemical Analysis of CD30+ Cells
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Formalin-fixed and paraffin-embedded (FFPE) biopsies of reactive lymph nodes for which parts were also available as frozen tissue were stained for CD30 with the anti-CD30 monoclonal antibody BerH2 (Dako, Hamburg, Germany) to identify lymph nodes with a substantial number of CD30+ cells. Five of the eight cases included in the IGHV gene analysis were further characterized by double stainings for CD30 (clone EP154, Diagnostic Biosystems, Pleasanton, CA, USA, at dilution 1:100) combined with staining for PAX5 (clone DAK-PAX5, Dako, at dilution 1:100), CD138 (clone MI15, Dako, at dilution 1:100), or MUM1/IRF4 (clone MUM1P, Dako, at dilution 1:100). The double stainings were performed using the Vecta Fluor Duet Double Labeling Kit DK-8828, Vector Laboratories, Newark, CA, USA.
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