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Mouse anti human cd28 antibody

Manufactured by BD

Mouse anti-human CD28 antibody is a laboratory reagent that specifically binds to the CD28 protein expressed on the surface of human T cells. CD28 is a co-stimulatory receptor that plays a crucial role in the activation and proliferation of T cells. This antibody can be used in various immunological applications, such as flow cytometry, cell sorting, and in vitro cell stimulation experiments.

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2 protocols using mouse anti human cd28 antibody

1

Activation of Human T Cells

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All chemicals not specifically mentioned are from Sigma-Aldrich (highest grade). The following antibodies or reagents were used: anti-LFA-1 (ITGAL) antibody (Antibodies-online), mouse anti-human CD28 antibody (BD Pharmingen), and mouse anti-human CD3 antibody (Diaclone).
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2

T Cell Activation Protocols

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T cells were activated using either 40.5 nM phorbol-12-myristate-13-acetate (PMA, EMD Millipore, Billerica, MA) supplemented by 1.5 μM ionomycin (Sigma-Aldrich), 2 μM thapsigargin (TG, Sigma-Aldrich) or 10 μg/ml mouse anti-human CD3 antibody (BD Biosciences, San Jose, CA, Cat # 555336) along with 10 μg/ml mouse anti-human CD28 antibody (BD Biosciences, Cat # 555726). The activation times for each activation protocol are mentioned in the individual figure legends.
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