1 2 dipalmitoyl sn glycero 3 phosphocholine

Manufactured by Echelon Biosciences

Sourced in United States

1,2-Dipalmitoyl-sn-glycero-3-phosphocholine is a synthetic phospholipid commonly used as a model compound for biological membranes. It is a structural component of cell membranes and plays a role in maintaining membrane integrity and function.

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Lab products found in correlation

Cholesterol, by Echelon Biosciences (1 mentions) 1 2 dioleoyl 3 trimethylammonium propane chloride salt dotap, by Avanti Polar Lipids (1 mentions) Triton x 100, by Samchun (1 mentions) Cholesterol, by Merck Group (1 mentions) Doxorubicin hydrochloride, by LC Laboratories (1 mentions) 1 2 dipalmitoyl 3 trimethylammonium propane chloride salt 16 0 tap, by Avanti Polar Lipids (1 mentions) Dichloromethane, by Daejung Chemicals (1 mentions) Clodronate liposomes, by Merck Group (1 mentions) C kit 2b8, by Thermo Fisher Scientific (1 mentions) Mouse igm mopc 104e, by BioLegend (1 mentions) Mouse igg1 mopc 21, by Merck Group (1 mentions) Anti flag m2, by Merck Group (1 mentions)

3 protocols using 1 2 dipalmitoyl sn glycero 3 phosphocholine

Formulation and Characterization of Lipid-Based Nanoparticles

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1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and cholesterol were purchased from Echelon Biosciences (Salt Lake City, UT, USA). 1,2-dioleoyl-3-trimethylammonium-propane (chloride salt) (DOTAP) was obtained from Avanti Polar Lipids (Alabaster, AL, USA). Metformin hydrochloride (Met) was purchased from Cayman Chemical Company (Ann Arbor, MI, USA). Phosphate Buffer saline (PBS) pH 7.5 and HPLC grade solvents—acetonitrile and water—were procured from Fisher Bioreagents (Fisher Sci, Hampton, NH, USA). Various assay kits and other molecular biology grade reagents were obtained from various sources, which have been listed along with their respective methods.

Shukla S.K., Kulkarni N.S., Chan A., Parvathaneni V., Farrales P., Muth A, & Gupta V. (2019). Metformin-Encapsulated Liposome Delivery System: An Effective Treatment Approach against Breast Cancer. Pharmaceutics, 11(11), 559.

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Liposomal Doxorubicin Synthesis and Characterization

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1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-Dipalmitoyl-sn-glycero-3-phosphoserine, sodium salt (DPPS) were purchased from Echelon Biosciences (Salt Lake City, UT, USA). 1,2-dipalmitoyl-3-trimethylammonium-propane [chloride salt (16:0 TAP)] was purchased from Avanti Polar Lipids (Birmingham, AL, USA). Cholesterol was purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). Dichloromethane was purchased from Daejung Chemical (Cheongwon, Korea). A dialysis membrane [pre-wetted RC Tubing (MWCO: 25 kD)] was purchased from Spectrum (Spectra/Por; Laguna Hills, CA, USA). Dimethyl sulfoxide, 99.0% (methyl sulfoxide, DMSO) and Triton X-100 were purchased from Samchun Pure Chemical (Pyeongtaek, Korea). Phosphate-buffered saline (PBS, pH 7.4) was purchased from Gibco (Thermo Fisher Scientific, Inc., Waltham, MA, USA). Doxorubicin hydrochloride was purchased from LC laboratories (Woburn, MA, USA).
Methods

Lee H., Son J., Na C.B., Yi G., Koo H, & Park J.B. (2018). The effects of doxorubicin-loaded liposomes on viability, stem cell surface marker expression and secretion of vascular endothelial growth factor of three-dimensional stem cell spheroids. Experimental and Therapeutic Medicine, 15(6), 4950-4960.

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Murine Immune Cell Phenotyping

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The following Abs were used: rat anti-LMIR3/CD300f (3–14–11) was obtained from ACTGen; anti-Flag (M2) and mouse IgG1 (MOPC21) were purchased from Sigma-Aldrich; fluorescein isothiocyanate (FITC)-conjugated anti-CD11b (M1/70), Gr-1 (RB6-8C5), F4/80 (BM8), or the high-affinity IgE receptor (FcεRI)-α (MAR-1), phycoerythrin (PE)-conjugated anti-Gr-1 (RB6-8C5), CD45.1 (A20), CD45.2 (104), Ly-6G (1A8), CD11b (M1/70), or c-kit (2B8) or streptavidin- or allophycocyanin (APC)-conjugated anti-c-kit (2B8) and rat IgG2a (eBR2a) were from eBioscience; anti-ceramide Ab (MID 15B4) was from Enzo Life Sciences, mouse IgM (MOPC-104E) was from BioLegend, rat IgG1/2a (G28-5) was from BD Biosciences. Clodronate liposomes were from Sigma-Aldrich. All the cytokines were obtained from R&D Systems. 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (phosphatidylcholine [PC]) and 1,2-Dipalmitoyl-sn-glycero-3-phosphoserine (phosphatidylserine [PS]) were from Echelon Biosciences Inc. All other reagents were from Sigma unless stated otherwise^{13 (link)}.

Izawa K., Maehara A., Isobe M., Yasuda Y., Urai M., Hoshino Y., Ueno K., Matsukawa T., Takahashi M., Kaitani A., Shiba E., Takamori A., Uchida S., Uchida K., Maeda K., Nakano N., Yamanishi Y., Oki T., Voehringer D., Roers A., Nakae S., Ishikawa J., Kinjo Y., Shimizu T., Ogawa H., Okumura K., Kitamura T, & Kitaura J. (2017). Disrupting ceramide-CD300f interaction prevents septic peritonitis by stimulating neutrophil recruitment. Scientific Reports, 7, 4298.

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