L glutamic acid

Manufactured by Cambridge Isotopes

L-glutamic acid is a non-essential amino acid that is commonly used in laboratory settings. It serves as a core component in various biochemical reactions and assays. The product provides a reliable source of this amino acid for researchers and scientists to utilize in their work.

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Lab products found in correlation

Protease inhibitor cocktail, by G Biosciences (1 mentions) L lysine, by Cambridge Isotopes (1 mentions) Protein assay dye regent, by Bio-Rad (1 mentions) L glutamine, by Cambridge Isotopes (1 mentions) L methionine, by Cambridge Isotopes (1 mentions) Aav8 tbg cre, by Addgene (1 mentions) 0.45 μm nitrocellulose membrane, by Bio-Rad (1 mentions) L tryptophan, by Cambridge Isotopes (1 mentions) C57bl 6j mice, by Jackson ImmunoResearch (1 mentions) N methyl n trimethylsilyl trifluoroacetamide, by Merck Group (1 mentions) L valine, by Cambridge Isotopes (1 mentions)

2 protocols using l glutamic acid

Metabolomic Analysis of Transgenic Mice

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C57bl6/j mice were obtained from Jackson Laboratories (Stock #000664). Labeled internal standards citric acid (2,2,4,4-D4, 98%; #DLM-3487), succinic acid (2,2,3,3-D4, 98%; #DLM-584), l-valine (2,3,4,4,4,5,5,5-D8; 98%; #DLM-311), l-glutamic acid (¹³C5, 99%; #CLM-1800), l-glutamine (¹³C5, 99%, #CLM-1822), l-lysine (¹³C6, 99%; #CLM-2247), l-methionine (¹³C5, 99%; #CLM-893), and l-tryptophan (¹³C11, 99%; #CLM-4290) and tracer glucose (¹³C6, 99%, #CLM-1396) were obtained from Cambridge Isotope Laboratories, Inc. AAV8-TBG-NULL (#105536-AAV8) and AAV8-TBG-Cre (#107787-AAV8) were purchased from Addgene. Pre-filled bead mill tubes containing 1.4 mm ceramic beads (#15-340-153) were purchased from FisherScientific. Methoxaymine hydrochloride (#226904, MOX) and N-methyl-N-(trimethylsilyl) trifluoroacetamide (#694709, MSTFA) were purchased from Sigma Aldrich. Protease inhibitor cocktail (#786-437) was purchased from G Biosciences. Bio-Rad protein assay dye regent (#5000006) and 0.45 μm nitrocellulose membrane (#1620115) were purchased from Bio-Rad.

Rauckhorst A.J., Borcherding N., Pape D.J., Kraus A.S., Scerbo D.A, & Taylor E.B. (2022). Mouse tissue harvest-induced hypoxia rapidly alters the in vivo metabolome, between-genotype metabolite level differences, and 13C-tracing enrichments. Molecular Metabolism, 66, 101596.

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Embryonic Tissue Analysis with Stable Isotopes

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Fluoxetine D6- oxalate (FLX) [# F919; 98 atom (at) % ²H], Carbamazepine (CBZ) [# DLM-2806-1.2; 98 at % ²H], Venlafaxine (VNX) [# V009; 98 at % ²H] and L-glutamic acid [# DLM-556-0.05; 98 at % ²H] were obtained from Cambridge Isotope Laboratories Inc. (MA). The overall experimental design comprised five treatments: FLX (concentration = 10μg/l; n=4), VNX (50μg/l; n=4), CBZ (100μg/l; n=4), control (no treatment; n=4) and negative control (contaminated with ²H-labeled L-glutamine; n=1). To examine the possibility of embryonic tissue contamination by maternal blood during dissection, we performed control experiments by bathing dissected embryos in phosphate buffer saline (PBS) containing 0.03 mg/ml ²H-labeled L-glutamine solution followed by 7 to 8 washes with PBS. Subsequent measurements of embryonic tissue resulted in no detection of ²H leading us to adopt the same method for experimental dissections.

Kaushik G., Huber D.P., Aho K., Finney B., Bearden S., Zarbalis K.S, & Thomas M.A. (2016). Maternal exposure to Carbamazepine at environmental concentrations can cross intestinal and placental barriers. Biochemical and biophysical research communications, 474(2), 291-295.

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