STAT3 mouse monoclonal antibody (catalog No. 9139), pSTAT3-Tyr705 rabbit monoclonal antibody (catalog No. 9145), pSTAT3-Ser-727 rabbit polyclonal antibody (catalog No. 9134), c-Fos rabbit monoclonal antibody (catalog No. 2250), and GAPDH rabbit monoclonal antibody (catalog No. 2118) were from Cell Signaling. c-Myc mouse monoclonal antibody (catalog No. sc-40) was from Santa Cruz, and IL-6 mouse monoclonal antibody (A3218) was from eBioscience. STAT3 inhibitor NSC74859 (also known as S3I-201) (catalog No. S1155) was from Selleck Chemicals. Primary antibodies were used at 1:1000 dilution, except GAPDH (1:5000). For the secondary antibodies, the dilution was 1:10,000. Protease (catalog No. 11697498001) and phosphatase (catalog No. P5726) inhibitors were from Sigma-Aldrich. Other chemical reagents were from Sigma-Aldrich.
Stat3 mouse monoclonal antibody
Manufactured by Cell Signaling Technology
Sourced in United States
The STAT3 mouse monoclonal antibody is a laboratory reagent produced by Cell Signaling Technology. It is designed to detect STAT3 protein expression in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
C myc mouse monoclonal antibody, by Santa Cruz Biotechnology (1 mentions)
Gapdh rabbit monoclonal antibody, by Cell Signaling Technology (1 mentions)
C fos rabbit monoclonal antibody, by Cell Signaling Technology (1 mentions)
Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions)
Anti p21 mouse monoclonal antibody, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti caspase 3 polyclonal antibody, by Abcam (1 mentions)
3 protocols using stat3 mouse monoclonal antibody
1
Western Blot Analysis of STAT3 Signaling Pathway
2
Melanoma and Lung Cancer Cell Culture
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B16-F10 cells (murine melanoma) were obtained as a gift from Dr Hemachand Tummala. NCI-H226 cells were obtained from the NCI. Both cell lines were maintained as monolayers and passaged as necessary in an RPMI 1640 growth medium (RPMI 1640 medium supplemented with 10% Fetal bovine serum [FBS] and 1% penicillin/streptomycin). RPMI 1640 medium, penicillin/streptomycin, phosphate-buffered saline (PBS), and Dulbecco phosphate-buffered saline (DPBS) were obtained from Mediatech (Herndon, VA, USA). Fetal bovine serum was purchased from Atlanta Biologicals (Lawrenceville, GA, USA). Anti–signal transducer and activator of transcription 3 (STAT3) mouse monoclonal antibody was purchased from Cell Signaling Technology (Danvers, MA, USA). Anti–caspase 3 rabbit polyclonal antibody was obtained from Abcam (Cambridge, MA, USA). Anti-p53 mouse monoclonal antibody was purchased from Zymed Laboratories (South San Francisco, CA, USA). Anti-p21 mouse monoclonal antibody was purchased from Santa Cruz Biotechnology (Dallas, TX, USA).
3
Curcumin Inhibits A431 Skin SCC Invasion
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A431 cells were inoculated into the culture bottle by 1×10 4 /cm 2 . After being adhereded, the medicines were added into the bottle respectively, according to the following group: the negative control group (0.1%DMSO) and curcumin (5, 10,15 umol/L) treatment group. After being incubated for 24h, the total protein of the cells were extracted. It was separated by 10% SDS-PAGE, then it DOI:http://dx.doi.org/10.7314/ APJCP.2015 APJCP. .16.7.2813 Inhibitory Effects of Curcumin on Invasion of A431 Skin SCC Cells was transferred onto PVDF membrane. The membrane was closed by 5% skim milk at room temperature for 1h. Then it was washed by TBST for 3 times(5min/ time). The first antibody of STAT3 mouse monoclonal antibody and P-STAT3 mouse monoclonal antibody (Cell Signaling Technologies company,USA)was added on the membrane, it was incubated at 4℃ overnight. It was washed by TBST for 3 times(5min/time) again. The secend antibody was added on the membrane, shaked for 1h at room temperature. Then it was washed by TBST for 3 times(5min/time). It was imaged by ECL imaging system.
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