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Recombinant ccl20

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Recombinant CCL20 is a chemokine protein produced using recombinant DNA technology. Chemokines are a family of small cytokines that play a role in the immune system and inflammation. CCL20 is involved in the recruitment and activation of specific types of immune cells.

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Lab products found in correlation

Cell observer, by Zeiss (2 mentions) Fetal calf serum (fcs), by Cambrex (1 mentions) Slide angiogenesis chamber, by Ibidi (1 mentions) Egm 2 mv, by Cambrex (1 mentions) Growth factor reduced matrigel, by BD (1 mentions)

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CCL20 (56 citations)

2 protocols using recombinant ccl20

1

Wound Healing Assay with CCL20

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BECs were cultured to confluence in 24-well plates. Scratch wounds were introduced in the monolayers by using a sterile 0.1–10-μl pipette tip. After washing away cells in suspension, cultures were supplied with cell medium containing 0 or 100 ng/ml recombinant CCL20 (R&D Systems). Imaging was performed with a Zeiss Cell Observer (Zeiss).
Hippe A., Braun S.A., Oláh P., Gerber P.A., Schorr A., Seeliger S., Holtz S., Jannasch K., Pivarcsi A., Buhren B., Schrumpf H., Kislat A., Bünemann E., Steinhoff M., Fischer J., Lira S.A., Boukamp P., Hevezi P., Stoecklein N.H., Hoffmann T., Alves F., Sleeman J., Bauer T., Klufa J., Amberg N., Sibilia M., Zlotnik A., Müller-Homey A, & Homey B. (2020). EGFR/Ras-induced CCL20 production modulates the tumour microenvironment. British Journal of Cancer, 123(6), 942-954.
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2

Angiogenesis Tube Formation Assay

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In total, 2 × 103 BECs were seeded in µ-slide angiogenesis chambers (Ibidi, Munich, Germany) on growth factor-reduced matrigel (BD Biosciences, San Jose, CA, USA). Cells were supplemented with 0, 10, 100 or 1000 ng/ml of recombinant CCL20 (R&D Systems) in EGM-2MV containing 5% FCS (Clonetics). Tube formation was imaged with a Zeiss Cell Observer (Zeiss).
Hippe A., Braun S.A., Oláh P., Gerber P.A., Schorr A., Seeliger S., Holtz S., Jannasch K., Pivarcsi A., Buhren B., Schrumpf H., Kislat A., Bünemann E., Steinhoff M., Fischer J., Lira S.A., Boukamp P., Hevezi P., Stoecklein N.H., Hoffmann T., Alves F., Sleeman J., Bauer T., Klufa J., Amberg N., Sibilia M., Zlotnik A., Müller-Homey A, & Homey B. (2020). EGFR/Ras-induced CCL20 production modulates the tumour microenvironment. British Journal of Cancer, 123(6), 942-954.
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