All reagents and solvents, unless specified otherwise, were purchased from Sigma Aldrich (Zwijndrecht, Netherlands) and VWR International (Amsterdam, Netherlands), and used without further purification. All materials were purchased from VWR International (Amsterdam, Netherlands), unless specified otherwise. Saline solution for injection (0.9% NaCl w/v%) and water for injection were acquired from B. Braun (Melsunger, Germany). The cassette was prepared in-house, starting from a commercially available [18F]FDOPA cassette from Trasis (Ans, Belgium). The GMP-grade precursor to [18F]DPA-714, DPA-714 tosylate, was purchased from Pharmasynth (Tallinn, Estonia). Sep-Pak Accell Plus QMA carbonate and Sep-Pak C18 Plus cartridges were purchased from Waters Corporation (Etten-Leur, Netherlands). Fluorine-18 for labelling was obtained on-site using a Siemens Eclipse HP cyclotron, in the form of [18O]H2O-bound [18F]fluoride via a 18O(p,n)18F nuclear reaction in a tantalum target. 18O-enriched water (≥97%) was purchased from Rotem Industries (Dimona, Israel). Cyclotron-specific parts are described in “Cyclotron ” section.
Water for injection
Manufactured by B. Braun
Sourced in Germany
Water for Injection is a sterile, highly purified water intended for use in the preparation of parenteral solutions and for other applications requiring a high-quality water source. It is produced through a multi-stage purification process to ensure its purity, quality, and suitability for pharmaceutical and medical applications.
Automatically generated - may contain errors
Lab products found in correlation
Sep pak accell plus qma carbonate, by Waters Corporation (1 mentions)
Sep pak plus c18 cartridge, by Waters Corporation (1 mentions)
Eclipse hp cyclotron, by Siemens (1 mentions)
Water for injection, by Merck Group (1 mentions)
Smoflipid, by Fresenius (1 mentions)
Glutamine, by Sartorius (1 mentions)
Streptomycin, by Sartorius (1 mentions)
Puromycin, by Santa Cruz Biotechnology (1 mentions)
Sodium pyruvate, by Sartorius (1 mentions)
6 protocols using water for injection
1
Radiosynthesis of [18F]DPA-714 PET Tracer
2
Cardioprotective Effects of D-PA
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The rats were randomly divided into six groups. Firstly, D-PA in the doses of 11 or 44 mg/kg or water for injection (B. Braun, Germany, 2 mL/kg) was administered into the tail vein. ISO (Sigma-Aldrich, 100 mg/kg, 50 mg/mL) or water for injection was given s.c. 5 min later. The groups in which rats received ISO are designated as ISO (positive control), D-PA11+ISO, and D-PA44+ISO, while the others are designated as C (negative control), D-PA11, and D-PA44.
3
Sucrose Formulation for Lyophilization
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Ph. Eur. certified D(+)-sucrose was purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany). Water for injection (B. Braun Melsungen AG, Melsungen, Germany) was used for the preparation of all solutions. TopLyo® serum tubing vials (20R and 10R) and standard serum tubing vials (4R) were obtained from Schott AG (Mainz, Germany). Westar® RS igloo stoppers were kindly provided by West Pharmaceuticals (Eschweiler, Germany). All chemicals were at least of analytical grade and used without further purification.
4
Magnolol-Lipid Emulsion Formulation
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Magnolol was purchased from Pol-Aura, Poland, and SMOFlipid was purchased from Fresenius Kabi AB, Sweden. Purified soybean oil 700 (Ph. Eur., USP), purified olive oil (Ph. Eur., USP), purified fish oil (Ph. Eur. Type I), and MCT (Ph. Eur., USP) were very kindly gifted by Lipoid GmbH (Ludwigshafen, Germany). Water for injection was purchased from B. Braun Melsungen AG, Melsungen, Germany. All organic solvents used in the studies were of analytical or high-performance liquid chromatographic grade. Epithelial human liver cells, THLE-2 (CRL-2706), were purchased from the American Type Culture Collection (ATCC), Manassas, VA, USA.
5
Freeze-Drying Vial Sublimation Study
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All vials were obtained from SGD S.A. (Puteaux, France). Three different types of molded vials with a nominal fill volume of 20 mL were used in this study: 20-mL vials manufactured by a BB process (“20-mL BB”), by a PB process with the same manufacturing mold as the BB vials (“20-mL PB1”), and by a PB process with from a freeze-drying perspective optimized geometrical features (“20-mL PB2”). Two 50-mL vials with different clear glass compositions manufactured by a PB process in the same molds (“50-mL PB1” and “50-mL PB2”) were analyzed. Additionally, 20-mL serum tubing vials were analyzed (“20-mL ST”) for comparison.
20 mm bromobutyl igloo stoppers by West Pharmaceutical Services (Eschweiler, Germany) and 32 mm bromobutyl cruciform stoppers by Datwyler Pharma Packaging International (Alken, Belgium) were used in the experiments. The sublimation experiments were performed with Water for Injection (WFI) by B Braun (Melsungen, Germany). Temperatures were monitored with calibrated 36 AWG thin-wire type T thermocouples (TCs) from OMEGA Engineering (Deckenpfronn, Germany). The vials were weighed on a calibrated XP205DR analytical balance (Mettler Toledo GmbH, Gießen, Germany).
20 mm bromobutyl igloo stoppers by West Pharmaceutical Services (Eschweiler, Germany) and 32 mm bromobutyl cruciform stoppers by Datwyler Pharma Packaging International (Alken, Belgium) were used in the experiments. The sublimation experiments were performed with Water for Injection (WFI) by B Braun (Melsungen, Germany). Temperatures were monitored with calibrated 36 AWG thin-wire type T thermocouples (TCs) from OMEGA Engineering (Deckenpfronn, Germany). The vials were weighed on a calibrated XP205DR analytical balance (Mettler Toledo GmbH, Gießen, Germany).
6
Branched PEG Hydrogel Synthesis
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Commercially available chemicals and reagents included the following:
Phosphate buffered saline (PBS, pH 7.4) was purchased from Sigma Aldrich, Rehovot, Israel.
Hank’s Balanced Salt Solution (HBSS), Fetal Bovine Serum (FBS), RPMI 1640 medium, 0.25% Trypsin-EDTA solution, sodium pyruvate, glutamine, and antibiotics (penicillin, streptomycin) were all from Biological Industries, Beit-Haemek, Israel. Puromycin was from Santa Cruz Biotechnology, Inc., TX, USA.
Amine functionalized 6-arm-branched, polyethylene glycol (PEG), 20 kDa molecular weight, was purchased from SunBio, South Korea. Water for injection (WFI) was from B. Braun Medical Inc., PA, USA.
Phosphate buffered saline (PBS, pH 7.4) was purchased from Sigma Aldrich, Rehovot, Israel.
Hank’s Balanced Salt Solution (HBSS), Fetal Bovine Serum (FBS), RPMI 1640 medium, 0.25% Trypsin-EDTA solution, sodium pyruvate, glutamine, and antibiotics (penicillin, streptomycin) were all from Biological Industries, Beit-Haemek, Israel. Puromycin was from Santa Cruz Biotechnology, Inc., TX, USA.
Amine functionalized 6-arm-branched, polyethylene glycol (PEG), 20 kDa molecular weight, was purchased from SunBio, South Korea. Water for injection (WFI) was from B. Braun Medical Inc., PA, USA.
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