Mouse retinal ganglion cells (RGCs) were obtain from Chinese Academy of Sciences; fetal calf serum (GIBCO, Rockville, MD, USA); penicillin and streptomycin (100×) (Solarbio, Beijing, China); 0.25% trypsin (Solarbio, Beijing, China); primary antibody MRE11, RAD50, NBS1 (Beyotime Biotechnology, Shanghai, China); CCK-8 (SAB); confocal microscope (Olyplus, Tokyo, Japan); enzyme label analyzer (Beijing Pulang New Technology Co., Ltd, Beijing, China); Western blot instruments (Eppendorf, Hamburg, Germany); flow cytometry (Thermo Fisher, Waltham, MA, USA).
Penicillin and streptomycin 100
Manufactured by Solarbio
Sourced in United States, China
Penicillin and streptomycin (100×) is a laboratory reagent used as a broad-spectrum antibiotic mixture. It is typically used to prevent bacterial contamination in cell culture media and other biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Flow cytometry, by Thermo Fisher Scientific (1 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions)
Western blot instruments, by Eppendorf (1 mentions)
Trypsin, by Solarbio (1 mentions)
Crystal violet stain solution, by Beyotime (1 mentions)
First and second antibodies, by Cell Signaling Technology (1 mentions)
Ifluor 488 conjugated phalloidin, by Abcam (1 mentions)
Sds page electrophoresis solution, by Beyotime (1 mentions)
Magnolol, by Selleck Chemicals (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Matrigel, by Corning (1 mentions)
Dulbecco s modified eagle s medium dmem, by Corning (1 mentions)
2 protocols using penicillin and streptomycin 100
1
Retinal Ganglion Cell Culture Protocol
2
Transwell Invasion Assay with Magnolol
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Dulbecco’s modified Eagle’s Medium (DMEM), Transwell nesting (with polycarbonate film 605 mm 8.0 μm), and Matrigel™ were purchased from Corning (NY, USA). Fetal bovine serum (FBS) was purchased from Tianhang (Huzhou, China). Dimethyl sulfoxide (DMSO) and 0.25% trypsin-EDTA (1X) were purchased from Sigma-Aldrich (Shanghai, China). The penicillin and streptomycin (100×) was purchased from Solarbio (Beijing, China). Magnolol and 5-FU were purchased from Selleck (Shanghai, China). Cell counting kit-8 (CCK-8), Phosphate buffer solution (PBS), Western transfer solution, SDS-PAGE electrophoresis solution and Crystal violet stain solution were purchased from Beyotime (Shanghai, China). iFluor 488-conjugated phalloidin was purchased from Abcam (Shanghai, China). First and second antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).
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