The CDS fragments of ATG5, ATG7, and ATG16 were inserted into the BamHI-SmaI site of pKD7 vector with 3× FLAG-tag (51 (link)). These fusion genes were transformed into Δclp1 expressing Clp1-GFP. Total protein was extracted and incubated with anti-GFP affinity beads 4FF SA070005 (Smart-Lifesciences, China) for 4 h at 4°C. After the beads were washed with low- and high-salt buffers, proteins were eluted with 0.2 M glycerol-HCl buffer (pH 3.3). Eluted and total proteins were detected by Western blotting with anti-GFP antibody and anti-FLAG antibody (HUABIO, China).
Anti gfp antibody and anti flag antibody
Manufactured by Huabio
Sourced in China
The anti-GFP antibody and anti-FLAG antibody are lab equipment used for the detection and purification of proteins. The anti-GFP antibody recognizes and binds to the Green Fluorescent Protein (GFP), while the anti-FLAG antibody recognizes and binds to the FLAG tag, a commonly used protein tag. These antibodies can be used in various techniques, such as Western blotting, immunoprecipitation, and immunofluorescence, to identify and isolate target proteins.
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Lab products found in correlation
2 protocols using anti gfp antibody and anti flag antibody
1
Affinity Purification of ATG Proteins
2
Affinity Purification of ATG Proteins
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The CDS fragments of ATG5, ATG7, and ATG16 were inserted into the BamHI-SmaI site of pKD7 vector with 3× FLAG-tag (51 (link)). These fusion genes were transformed into Δclp1 expressing Clp1-GFP. Total protein was extracted and incubated with anti-GFP affinity beads 4FF SA070005 (Smart-Lifesciences, China) for 4 h at 4°C. After the beads were washed with low- and high-salt buffers, proteins were eluted with 0.2 M glycerol-HCl buffer (pH 3.3). Eluted and total proteins were detected by Western blotting with anti-GFP antibody and anti-FLAG antibody (HUABIO, China).
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