A375 cells mounted on cover slips were treated with IFNγ for 24 hours. After washing 2 times with PBS, cells were fixed with 4% PFA for 15 minutes and washed 2 times with PBS for 5 minutes each. Then, the cells were permeabilized with 0.5% triton x-100 in PBS for 10 minutes and rinsed 2 times with PBS for 5 minutes each. Antigens were blocked with 10% normal goal serum in PBS for 30 minutes. Then, primary antibodies were added at 1:50 dilutions of mouse anti-human GBP1-5 antibody (Santa Cruz, 166960) or rabbit anti-human HSP90 antibody (CST, 4877), and incubated at 4°C overnight. Afterwards, the cells were washed and incubated with 1:500 dilutions of Qdot 605 labeled secondary goat anti-mouse antibody (Thermo Fisher, Q11002) or AF488-labeled secondary goat anti-rabbit antibody (Thermo Fisher, A11034), and then mounted on glass slides with ProLong Gold reagent containing DAPI. Confocal fluorescence images were collected using a 63X oil-immersion objective (Leica SP5 Inverted 2-Photon FLIM confocal).
Qdot 605 labeled secondary goat anti mouse antibody
Manufactured by Thermo Fisher Scientific
The Qdot 605 labeled secondary goat anti-mouse antibody is a fluorescent-labeled secondary antibody used for detection and visualization of mouse primary antibodies in various applications, such as immunohistochemistry, flow cytometry, and Western blotting. The Qdot 605 dye provides a bright, photostable signal for sensitive detection.
Automatically generated - may contain errors
2 protocols using qdot 605 labeled secondary goat anti mouse antibody
1
Immunofluorescence Imaging of GBP1-5 and HSP90
2
Immunofluorescence Imaging of GBP1-5 and HSP90
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