Immunohistochemistry staining was performed using a Discovery Ultra Ventana instrument (Roche), with the staining module of RUO Discovery Multimer V2 (V0.00.0083). Slides were first incubated in blocking agent containing 2% BSA PBS, and Discovery Ultra antibody block (Cat # 760–4204) (Roche). Slides were incubated with primary antibodies for 60 minutes at 37°C. The following primary antibodies were used: anti-phospho-STAT1 (Tyr701) 58D6 (Cell Signaling) and anti-CD68 Clone Kp1 (Abcam) at recommended dilution in blocking agent. The slides were then incubated with Omni Map anti-rabbit HRP-conjugated secondary antibody (Multimer HRP) (Cat # 760–4311) (Roche) for 32 minutes. Positive signals were detected with the Discovery ChromoMap DAB Kit (Cat #760–159) (Roche).
Anti cd68 clone kp1
Manufactured by Abcam
Anti-CD68 Clone Kp1 is an antibody that binds to the CD68 protein, which is expressed on the surface of macrophages and monocytes. This antibody can be used for the identification and quantification of these cell types in various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti phospho stat1 tyr701 58d6, by Cell Signaling Technology (2 mentions)
Discovery ultra antibody block, by Roche (2 mentions)
Omni map anti rabbit hrp conjugated secondary antibody multimer hrp, by Roche (2 mentions)
Ventana discovery ultra, by Roche (2 mentions)
Discovery chromomap dab kit, by Roche (2 mentions)
2 protocols using anti cd68 clone kp1
1
Immunohistochemical Analysis of Phospho-STAT1 and CD68
2
Immunohistochemical Analysis of Phospho-STAT1 and CD68
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Immunohistochemistry staining was performed using a Discovery Ultra Ventana instrument (Roche), with the staining module of RUO Discovery Multimer V2 (V0.00.0083). Slides were first incubated in blocking agent containing 2% BSA PBS, and Discovery Ultra antibody block (Cat # 760–4204) (Roche). Slides were incubated with primary antibodies for 60 minutes at 37°C. The following primary antibodies were used: anti-phospho-STAT1 (Tyr701) 58D6 (Cell Signaling) and anti-CD68 Clone Kp1 (Abcam) at recommended dilution in blocking agent. The slides were then incubated with Omni Map anti-rabbit HRP-conjugated secondary antibody (Multimer HRP) (Cat # 760–4311) (Roche) for 32 minutes. Positive signals were detected with the Discovery ChromoMap DAB Kit (Cat #760–159) (Roche).
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