Digital BD LSR II flow cytometry (BD Biosciences, Franklin Lakes, NJ) was used to evaluate apoptosis and cell cycle progression. Collected cells were mixed with binding buffer and 5 μL fluorescein isothiocyanate-labeled annexin V and 5 μL propidium iodide (BD Biosciences) to examine apoptosis. To evaluate cell cycle progression, we preserved the cells in 75% alcohol for 24 hours and mixed with cell cycle liquid (BD Biosciences).
Fluorescein isothiocyanate labeled annexin 5
Manufactured by BD
Sourced in United States
Fluorescein isothiocyanate-labeled annexin V is a fluorescent protein that binds to phosphatidylserine. It is commonly used in flow cytometry and fluorescence microscopy applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fluorescein isothiocyanate labeled annexin 5
1
Flow Cytometry Analysis of Apoptosis and Cell Cycle
2
Annexin V Apoptosis Assay in ACHN Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ACHN cells were inoculated on a 6-well plate at 1 x 10 6 cells/well, transfected for 24 h, washed twice with PBS, and resuspended with 1X Binding Buffer. Fluorescein isothiocyanate-labeled annexin V (50 mL; BD Biosciences, USA) and propidium iodide (50 mL) were added, gentle mixed, and stained at room temperature away from light for 15 min. Apoptosis was detected by flow cytometry.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!