Labrafil 1944

CRV431 was synthesized in-house to a purity of 97.3 % by modification of CsA and stored at 5°C. CsA was obtained from IVAX (Opava, Czech Republic). Lauroglycol 90, Labrasol, Labrafil M 2125, Labrafil 1944, Pecol, Labrafac WL, Capryol 90, Maisine CC, and Transcutol were purchased from Gattefosse (Montreal, Canada). Propylene glycol, filtered water, acetonitrile, methanol, scintillation vials and 12x75 ml borosilicate test tubes were purchased from Fischer Scientific (Pittsburgh, USA). Anhydrous ethanol was purchased from Commercial Alcohols (Toronto, Canada). Vitamin E, Tween 80, Tween 40, Tween 20, castor oil, dimethyl sulfoxide, Chremophor EL and Chremophor RH 40 were purchased from Sigma-Aldrich (St. Louis, USA). Span 80 was purchased from EMD Millipore Corporation (Burlington, USA). PEG 400 was purchased from BDH Incorporated (Mississauga, Canada).

EFV API was kindly donated by Lupin Research Park, Pvt. Ltd., Pune. Labrafil M 2125 CS, Labrafil 1944, Labrafac, Peceol, Labrasol, Transcutol®P, Lauroglycol, Caproyl 90, and Caproyl P were kindly gifted from Gattefosse (Gennevilliers, France). Capmul, Captex, and Caproyl PGE were kindly gifted from Abitec, USA. All other chemicals and reagents were of analytical grade.