Spb 1 fused silica capillary column

At the baseline of the CuVIC Trial, blood samples were obtained and LDL-C levels were determined using the Friedewald equation. Alongside routine laboratory tests, including lipid profiling at each participating center, samples were sent to Medical and Biological Laboratories, Co., LTD., Nagoya, Japan. At this facility, measurements were performed to assess levels of apolipoprotein A1 (Apo A1), apolipoprotein B (Apo B), high-sensitive C-reactive protein (hs-CRP), and malondialdehyde modified LDL (MDA-LDL). The non-cholesterol sterols (campesterol, sitosterol, and lathosterol) were measured at SRL, Inc., Tokyo, Japan, using gas chromatography (GC-2010, Shimadzu, Co., Kyoto, Japan). Oxysterols were measured using gas chromatography mass spectrometry (GC/MS QP2010; Shimadzu Co.) equipped with an SPB-1 fused silica capillary column (60 m × 0.25 mm, 0.25 μm phase thickness; Supelco Inc., Bellefonte, PA, USA). Participants provided information regarding their general demographic and health information at the time of enrollment, including sex, age, height, weight, smoking status, history of metabolic syndrome, hypertension, diabetes, dyslipidemia, and current use of medications such as statins, antihypertensive drugs, diabetes drugs, and insulin.

Lipids were extracted using the Bligh and Dyer method^{42 (link)}. For cholesterol analysis, the lipids were saponified at 70 °C for 1 h. Following the saponification, the sample solution was reacted with a trimethylsilylating agent at room temperature for 30 min. The derivatized were quantified by using a GC/MS QP2010 (Shimadzu, Kyoto, Japan) equipped with an SPB-1 fused silica capillary column of 60 m × 0.25 mm and 0.25 μm phase thickness (Supelco Inc., Bellefonte, PA, USA). The temperature program was initiated at 180 °C for 1 min, 20 °C/min at 250 °C, and then 5 °C/min at 290 °C and held for 30 min. The injection temperature was set at 250 °C, the interface at 250 °C, and the ion source adjusted to 200 °C. Quantification was performed using the selected ion monitoring (SIM) mode, with ions observed at m/z 329, 368, and 458 for cholesterol (quantification ions are underlined).