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Direct zol rna miniprep plus assay kit

Manufactured by Zymo Research
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The Direct-zol™ RNA Miniprep Plus assay kit is a laboratory product designed for the isolation and purification of total ribonucleic acid (RNA) from various biological samples. The kit utilizes a fast and efficient spin-column-based method to extract high-quality RNA suitable for downstream applications such as reverse transcription and real-time PCR.

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Lab products found in correlation

Ncounter mouse myeloid innate immunity v2 panel, by NanoString (2 mentions) Nd 1000, by Thermo Fisher Scientific (2 mentions)

2 protocols using direct zol rna miniprep plus assay kit

1

Analyzing Myeloid Immune Gene Expression in Frozen Lung Tissue

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Frozen lung tissues from −80°C were used for RNA isolation. Lung tissue (~100 mg) per sample was homogenized in Trizol for 20s. The RNA was isolated using the Direct-zol™ RNA Miniprep Plus assay kit (Cat# R2073, Zymo Research). Isolated RNA was quantified and checked for quality by spectrophotometer (ND-1000, NanoDrop Technologies, Wilmington, DE, USA). RNA samples were aliquoted and sent out to Nanostring facility for analysis of genes using nCounter Mouse Myeloid Innate Immunity v2 Panel (NanoString Technologies, Inc. Cat# XT-CSO-MMII2–12).
Wang Q., Sundar I., Li D., Lucas J., Muthumalage T., McDonough S, & Rahman I. (2020). E-cigarette-Induced Pulmonary Inflammation and Dysregulated Repair are Mediated by nAChR α7 Receptor: Role of nAChR α7 in ACE2 Covid-19 receptor regulation. Research Square.
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2

Lung Tissue RNA Isolation and Analysis

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Frozen lung tissues from − 80 °C were used for RNA isolation. Lung tissue (~ 100 mg per sample was homogenized in Trizol for 20s. The RNA was isolated using the Direct-zol™ RNA Miniprep Plus assay kit (Cat# R2073, Zymo Research). Isolated RNA was quantified and checked for quality by spectrophotometer (ND-1000, NanoDrop Technologies, Wilmington, DE, USA). RNA samples were aliquoted and sent out to Nanostring facility for analysis of genes using nCounter Mouse Myeloid Innate Immunity v2 Panel (NanoString Technologies, Inc. Cat# XT-CSO-MMII2–12).
Wang Q., Sundar I.K., Li D., Lucas J.H., Muthumalage T., McDonough S.R, & Rahman I. (2020). E-cigarette-induced pulmonary inflammation and dysregulated repair are mediated by nAChR α7 receptor: role of nAChR α7 in SARS-CoV-2 Covid-19 ACE2 receptor regulation. Respiratory Research, 21, 154.
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