Data presented are average of biological replicates. The numbers of biological replicates considered for each experiment are mentioned in the legend for respective figure. Error bars represent ± SEM. Significant differences among treatments were determined by one-way ANOVA and applying the least-significant difference post-hoc Tukey's test (p < 0.05) (SigmaPlot 11.0, Systat Software Inc., California, USA). The test of significance applied in RT-qPCR analysis was Student's t-test (p < 0.05) (SigmaPlot 11.0, Systat Software Inc., California, USA).
Sigmaplot 11
Manufactured by Grafiti LLC
Sourced in United States, Germany, United Kingdom, Uruguay
SigmaPlot 11.0 is a data analysis and graphing software. It is used for creating high-quality scientific and technical graphs and plots from data.
Automatically generated - may contain errors
Lab products found in correlation
Prism 5, by GraphPad (21 mentions)
Prism 6, by GraphPad (21 mentions)
Statistica 12, by StatSoft (15 mentions)
Sas 9, by SAS Institute (11 mentions)
Clampfit 10, by Molecular Devices (10 mentions)
Prism 8, by GraphPad (9 mentions)
Sas version 9, by SAS Institute (8 mentions)
Matlab, by MathWorks (7 mentions)
Prism 7, by GraphPad (7 mentions)
Statistix version 9, by SAS Institute (6 mentions)
Stata 13, by StataCorp (5 mentions)
Sigmastat 3, by Grafiti LLC (4 mentions)
Prism 3, by GraphPad (4 mentions)
Sas stat, by SAS Institute (4 mentions)
Statview 5.0j, by SAS Institute (4 mentions)
Prism 4, by GraphPad (3 mentions)
Prism 9, by GraphPad (3 mentions)
Spss statistics 22, by IBM (3 mentions)
Sas stat 9, by SAS Institute (3 mentions)
Spss 21, by IBM (3 mentions)
1 175 protocols using sigmaplot 11
1
Reproducible Biological Replicates Analysis
2
Comparative Analysis of Gene Expression
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For comparing two different culture conditions, hFF (n = 9) and suspension (n = 7) when using TLDA arrays, the unpaired t-test was used with a two-tailed p value (Prism 6, GraphPad Software, Inc., CA, USA). For comparing all cell lines and culture conditions separately, one-way ANOVA was used with the Bonferroni multiple comparison test (Prism 6, GraphPad Software, Inc., CA, USA). Levels of gene expression are expressed as means ± standard deviations (SDs). One-Way RM ANOVA (SigmaPlot 11.0; Systat Software Inc., CA, USA) was used to compare values under the different experimental conditions. Following the Shapiro–Wilk test for normality, pair-wise multiple comparisons were performed with Holm–Sidak or Dunnett’s procedure (SigmaPlot 11.0; Systat Software Inc.; CA, USA), as indicated in the figure legends. A p-value of ≤ 0.05 was considered statistically significant.
3
Gene Expression and Testosterone Regulation
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Gene expression, apoptotic frequency, and testosterone production were calculated as the means ± standard deviations (SD) for the triplicates run under each condition. Student’s t-test, One-way ANOVA and One-way RM ANOVA were applied to compare the differences between experimental conditions (SigmaPlot 11.0; Systat Software Inc., CA, USA). Following the Shapiro–Wilk test for normality, pairwise multiple comparisons were performed with the “Holm–Sidak” procedure as stated in the Figure legends (SigmaPlot 11.0; Systat Software Inc.). A difference was considered to be statistically significant if the p value was ≤0.05.
4
Evaluating Supersaturation of ABZ Formulations
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BioGIT data collected with ABZ free base formulations were evaluated versus recently reported intraluminal data after administration of ABZ free base formulations. 11 (link) Intraluminal data are presented as box-whisker plots showing the mean value, the median value, the 10th, 25th, 75th, and 90th percentiles, and the individual outlying data points.
Supersaturation in duodenal compartment was evaluated by estimating the degree of supersaturation (DS):
DS > 1 indicates supersaturation, DS ¼ 1 indicates saturation, and DS < 1 indicates unsaturated solution.
A 1-sample t-test (Sigmaplot 11.0, Systat Software, Inc., San Jose, CA) was applied for evaluating whether a sample mean DS value comes from a population with mean of 1. Differences between solubility in human fluids and biorelevant media and between ABZ free base and ABZ sulfate were evaluated with an unpaired t-test (Sigmaplot 11.0, Systat Software, Inc., San Jose, CA). Type I error was set at 0.05, in all cases.
Supersaturation in duodenal compartment was evaluated by estimating the degree of supersaturation (DS):
DS > 1 indicates supersaturation, DS ¼ 1 indicates saturation, and DS < 1 indicates unsaturated solution.
A 1-sample t-test (Sigmaplot 11.0, Systat Software, Inc., San Jose, CA) was applied for evaluating whether a sample mean DS value comes from a population with mean of 1. Differences between solubility in human fluids and biorelevant media and between ABZ free base and ABZ sulfate were evaluated with an unpaired t-test (Sigmaplot 11.0, Systat Software, Inc., San Jose, CA). Type I error was set at 0.05, in all cases.
5
Adhesive Bond Strength and Penetration
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Data obtained by bond strength (MPa) and penetration depth (%) were analyzed by 2-way ANOVA (application technique vs. conditioning protocols) and Tukey post-test (p=0.05). The Sigma Plot 11 software (Systat Software, San Jose, California, USA) was used for statistical analysis. Data from the fracture modes were qualitatively analyzed.
6
Germination and Polyphenol Analysis of Genotypes
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Total germination percentages, after arcsine transformation, and total polyphenols content were analysed by a randomized two-way analysis (main factors: genotypes and seed treatments) of variance (ANOVA) using COSTAT version 6.003 (cohort 3 Software). Data were arranged in a complete randomized experimental design with three replications. When the F test of the ANOVA was significant, treatment means were separated at p ≤ 0.05 according to the Least Significant Difference (LSD) method (Snedecor and Cochran 1989) . On the treatments achieving at least 50% germination, the time (hours) to 50% germination (t50) was calculated by a logistic equation with three parameters (SIGMAPLOT 11 software, Systat Software Inc., San Jose, California, USA):
where a is the maximum value of germination observed for each genotype, x is the time after seed imbibition, expressed in hours, x 0 is the time (hours) to 50% seed germination, b is a fitting parameter of the curve. The x value on the curve, which corresponded to 50% germination, was assumed as theoretical time (t50) to 50% germination (Patanè et al. 2016) (link). The confidence intervals of t50 were calculated on the 95% confidence bands of the logistic curve (SIGMA-PLOT 11 software).
where a is the maximum value of germination observed for each genotype, x is the time after seed imbibition, expressed in hours, x 0 is the time (hours) to 50% seed germination, b is a fitting parameter of the curve. The x value on the curve, which corresponded to 50% germination, was assumed as theoretical time (t50) to 50% germination (Patanè et al. 2016) (link). The confidence intervals of t50 were calculated on the 95% confidence bands of the logistic curve (SIGMA-PLOT 11 software).
7
Cocaine Generalization and Response Rate Analysis
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Graphical presentation of all data depict mean ±SEM. Response distribution data are expressed as percent cocaine-appropriate responding, which is the number of responses emitted on the cocaine-trained lever as a percentage of the total number of responses emitted. Response rate is the total number of responses divided by the time elapsed (in seconds) during the test session. Full generalization was said to occur if the group mean was significantly different (via one way ANOVA, followed by pairwise comparisons using the Holm-Sidak method) from saline and > 80% of the total responses were made on the cocaine-appropriate lever. Response rates were considered significantly different from control if the group mean was significantly different (via one way ANOVA, followed by pairwise comparisons using the Holm-Sidak method) from rates during the saline test session.
Potency data represent the mean ED50± 95% confidence intervals. ED50 values were considered statistically different in cases where the confidence intervals were nonoverlapping. SigmaPlot 11 software (Systat Software, San Jose, CA, USA) was used to generate figures, interpolate ED50 values, and conduct statistical analyses.
Potency data represent the mean ED50± 95% confidence intervals. ED50 values were considered statistically different in cases where the confidence intervals were nonoverlapping. SigmaPlot 11 software (Systat Software, San Jose, CA, USA) was used to generate figures, interpolate ED50 values, and conduct statistical analyses.
8
Statistical Analysis of Experimental Data
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SigmaPlot® 11 software (Systat Software Inc., Chicago, IL, USA) was used for the statistical analyses. Parametric analysis was performed by Student’s t-test. A p-value <0.05 was considered statistically significant.
9
Cocaine Generalization and Response Rate Analysis
10
Survival Analysis Protocol for DNA-PKcs Mutant
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