The cells were identified as MSCs by flow cytometry (FACSAria; BD Biosciences, Erembodegem, Belgium) at passage 1 to confirm the MSC immunophenotype of the cells. Cells were single stained using monoclonal antibodies against CD3-PE, CD14-PE-Cy7, CD19-PE-Cy7, CD45R0-APC, CD54-FITC, CD73-PE, CD90-APC (BD Biosciences, Franklin Lakes, NJ, USA), CD11a-APC, CD80-PE, CD86-PE, CD105-PE (R&D Systems, Minneapolis, MN, USA), CD34-APC, and HLA-DR-PE (ImmunoTools, Friesoythe, Germany). The FACS analysis was performed on 10,000 cells per sample and positive expression was defined as fluorescence level greater than 99% of the comparable unstained cell sample.
Cd34 apc
Manufactured by Immunotools
Sourced in United States, Germany
CD34-APC is a fluorescent-labeled antibody that binds to the CD34 antigen, which is expressed on hematopoietic stem and progenitor cells. It can be used for the identification and enumeration of CD34-positive cells in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Cd90 apc, by BD (6 mentions)
Cd73 pe, by BD (6 mentions)
Cd105 pe, by R&D Systems (6 mentions)
Hla dr pe, by Immunotools (6 mentions)
Facsaria, by BD (5 mentions)
Cd11a apc, by R&D Systems (4 mentions)
Cd14 pe cy7, by BD (3 mentions)
Cd19 pe cy7, by BD (3 mentions)
Cd45ro apc, by BD (3 mentions)
Cd86 pe, by R&D Systems (2 mentions)
Cd3 pe, by BD (1 mentions)
Cd54 fitc, by BD (1 mentions)
Cd146 pe, by BD (1 mentions)
Cd45 fitc, by Miltenyi Biotec (1 mentions)
Cd166 pe, by BD (1 mentions)
Cd49d pe, by BD (1 mentions)
Cd106 pe cy5, by BD (1 mentions)
Hla abc pe, by Immunotools (1 mentions)
Cd44 fitc, by Immunotools (1 mentions)
Alpha mem, by Thermo Fisher Scientific (1 mentions)
6 protocols using cd34 apc
1
Characterization of Mesenchymal Stem Cells
2
Multiparametric Characterization of Mesenchymal Stem Cells
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ASCs were further expanded in vitro and were analyzed at passages 4 to 5 by flow cytometry (FACSAria; BD Biosciences, Erembodegem, Belgium). Monoclonal antibodies against CD9‐PE, CD10‐PECy7, CD13‐PE, CD14‐PECy, CD19‐PECy7, CD29‐APC, CD49d‐PE, CD73‐PE, CD90‐APC, CD106‐PE‐Cy5, CD146‐PE, and CD166‐PE (BD Biosciences); CD45‐FITC (Miltenyi Biotech, Bergisch Gladbach, Germany); CD31‐FITC, CD34‐APC, CD44‐FITC, HLA‐ABC‐PE, and HLA‐DR‐PE (Immunotools GmbH, Friesoythe, Germany); and CD105‐PE (R&D Systems Inc., Minneapolis, Minnesota) were used. Analysis was performed on 10,000 cells per sample. The positive expression was defined as the level of fluorescence greater than 99% of the corresponding unstained cell sample.
3
Immunophenotyping of Human Adipose Stem Cells
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The hASCs used in the study were characterized by flow cytometry (FACSAria; BD Biosciences, Erembodegem, Belgium) at passage 1 to evaluate their immunophenotype. Human ASCs (10 000 cells/sample) were single stained with monoclonal antibodies: CD14-PE-Cy7, CD19-PE-Cy7, CD45RO-APC, CD73-PE, CD90-APC (aforementioned antibodies from BD Biosciences, Franklin Lakes, NJ, USA), CD11a-APC, CD105-PE (R&D Systems Inc., Minneapolis, MN, USA), CD34-APC, and HLA-DR-PE (Immunotools GmbH, Friesoythe, Germany). Fluorescence level greater than 99% was considered positive.
4
Immunophenotyping of Adipose-Derived Stem Cells
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ASCs expanded in standard conditions and under MMC in FBS, HS, and SF/XF were analyzed using flow cytometry in passage 4 (FACSAria; BD Biosciences, Erembodegem, Belgium) to determine the immunophenotype of the cells. Monoclonal antibodies (MAbs) against CD11a-allophycocyanin (APC), CD80-phycoerythrin (PE), CD86-PE, CD105-PE (R&D Systems Inc., Minneapolis, MN, USA), CD3 (PE), CD14-phycoerythrin-cyanine (PECy7), CD19-PECy7, CD45RO-APC, CD54-fluorescein isothiocyanate (FITC), CD73-PE, CD90-APC (BD Biosciences), CD34-APC, and HLA-DR-PE (ImmunoTools GmbH, Friesoythe, Germany) were used. Analysis was performed on 10000 cells per sample, and unstained cell samples were used to correct for background autofluorescence [32 (link)].
5
Characterization of RESSTORE01 Adipose Stem Cells
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The adipose tissue stem cell line RESSTORE01 (Master Cell Bank/Stock n°1—Donor RESSSTORE01, Batch n°: 591133643763) was cultured in the growth medium Alpha MEM (Gibco, Life technologies) supplemented with 5% human platelet lysate (Stemulate, Cook Medical, USA) and 1% Penicillin-Streptomycin (Lonza, Belgium). The medium was changed twice each week and cells were passaged when they reached 100% confluence. The cells were detached with TrypLE Select (Life Technologies™, Thermo Fisher Scientific) for 10 min at 37°C and then centrifuged at 1,000 rpm for 5 min. The RESSTORE01 cell phenotype was analyzed with flow cytometry (FACSAria Fusion Cell Sorter, BD Biosciences) at passage PX +1. Monoclonal antibodies against CD19-phycoerythrincyanine (PE-Cy7), CD45RO-allophycocyanin (APC), CD73-PE, CD90-APC (BD Biosciences), CD11a-APC, CD105-PE (R&D Systems Inc., Minneapolis, MN, USA), CD34-APC and HLA-DR-PE (Immunotools GmbH, Friesoythe, Germany) were used. The cells expressed (>95%) surface markers CD73, CD90, and CD105 and lacked the expression (<2%) of CD11a, CD19, CD34, CD45, and HLA-DR. Cells at passages PX +2/3 were used for the study.
6
Immunophenotyping of hASC and hBMSC
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The immunophenotype of hASC and hBMSC was analyzed by flow cytometry (FACSAria; BD Biosciences, Erembodegem, Belgium) at passage one. Cell samples (10,000 cells/sample) were single stained with the following monoclonal antibodies: CD14-PE-Cy7, CD19-PE-Cy7, CD45RO-APC, CD73-PE, CD90-APC (antibodies from BD Biosciences, Franklin Lakes, NJ, USA), CD11a-APC, CD105-PE (R&D Systems Inc., Minneapolis, MN, USA), CD34-APC, and HLA-DR-PE (Immunotools GmbH, Friesoythe, Germany). A fluorescence level greater than 99% was considered positive.
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