Log In Sign up
The largest database of trusted experimental protocols

Apc 036

Manufactured by Alomone
Visit Supplier
Sourced in Israel

APC-036 is a biochemical product used for research purposes. It functions as an antibody to aid in the detection and study of its target molecule.

Automatically generated - may contain errors

Lab products found in correlation

Ripa buffer, by Thermo Fisher Scientific (2 mentions) Ab10135, by Abcam (1 mentions) Ab99046, by Abcam (1 mentions) Ab228874, by Abcam (1 mentions) Ab5694, by Abcam (1 mentions) Ab110304, by Abcam (1 mentions) B14001, by Selleck Chemicals (1 mentions) Ab20272, by Abcam (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Apc 021, by Alomone (1 mentions)

2 protocols using apc 036

1

Antibody Validation for BKCa Channels

Check if the same lab product or an alternative is used in the 5 most similar protocols
An antibody against BKCa-α (ab99046) used for immunohistochemical staining and western blot was purchased from abcam (Cambridge, UK). An antibody against BKCa-β1 (APC-036) used for immunohistochemical staining and western blot was purchased from Alomone labs (Jerusalem, Israel). Antibodies against α-SMA (ab5694), SM22α (ab10135), and eIF5 (ab228874) used for western blot were purchased from abcam (Cambridge, UK).
Wang M., Li S., Liu H., Liu M., Zhang J., Wu Y., Xiao C, & Huang H. (2022). Large-conductance Ca2 +-activated K+ channel β1-subunit maintains the contractile phenotype of vascular smooth muscle cells. Frontiers in Cardiovascular Medicine, 9, 1062695.
+ Open protocol
+ Expand
2

Quantifying Protein Expression in Rat Coronary Arteries

Check if the same lab product or an alternative is used in the 5 most similar protocols
The rat coronary arterial fragments or hcSMCs were lysed in RIPA buffer (89901, Thermo scientific), which contained protease inhibitors (B14001, Bimake). The lysates were fractionated using SDS-PAGE and transferred onto polyvinylidene di-fluoride (PVDF) membranes (Millipore). The PVDF membranes were incubated with specific primary antibodies, with β-actin (ab20272, Abcam, 1:5000), BK-α (APC-021, Alomone Labs, 1:500), BK-β1 (APC-036, Alomone Labs, 1:1000), Sirt1 (ab110304, Abcam, 1:2000) and Nrf2 (SAB5700720, Sigma, 1:1000). Western blot bands were quantified using densitometry and ImageJ software. The density was normalized to the control treatment level, and the relative folds were normalized to the β-actin level.
Kong Q., Qian L.L., Zhang L., Liu H.H., Yang F., Zhang X.L., Wang C., Zhao X.X., Li K.L, & Wang R.X. (2024). Empagliflozin Induces Vascular Relaxation in Rat Coronary Artery Due to Activation of BK Channels. Diabetes, Metabolic Syndrome and Obesity, 17, 247-257.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!