Log In Sign up
The largest database of trusted experimental protocols

C jun

Manufactured by GenePharma
Visit Supplier
Sourced in China

The C-JUN is a laboratory equipment designed for gene expression analysis. It serves as a tool to quantify the expression levels of the c-Jun gene, which is a key regulator of cellular processes. The C-JUN equipment provides accurate and reliable measurements of c-Jun expression, enabling researchers to study its role in various biological systems.

Automatically generated - may contain errors

Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

C-Jun siRNA

2 protocols using c jun

1

c-JUN siRNA Transfection and Knockdown

Check if the same lab product or an alternative is used in the 5 most similar protocols
c-JUN siRNA oligonucleotides c-JUN(1)(sense, 5′-GCAAAGAUGGAAACGACCU-3′), c-JUN(2) (sense, 5′-CCUUCUAUGACGAUGCCCU-3′), and c-JUN(3) (sense, 5′-GAUGGAAACGACCUUCUAU-3′) were synthesized by GenePharma (Shanghai, China). The cells were harvested 48 h after transfection. Western blot assay was used to evaluate the RNAi effect.
Li R., He Q., Han S., Zhang M., Liu J., Su M., Wei S., Wang X, & Shen L. (2016). MBD3 inhibits formation of liver cancer stem cells. Oncotarget, 8(4), 6067-6078.
+ Open protocol
+ Expand
2

Silencing IGFBP3 and c-Jun in GBM

Check if the same lab product or an alternative is used in the 5 most similar protocols
Negative control siRNA and siRNA targeting at either of IGFBP3 and c-Jun (Genepharma, Shanghai, China) were transfected into EGFRvIII-expressing GBM cells or vector cells using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. si-Scr, sense: (5'-UAACGACGCGACGACGUAA-3'); si-IGFBP3, sense: (5'-CAGAGCACAGAUACCCAGAACUUCU-3'); si-c-Jun, sense: (5'-UGGGAGAGGCAUCAUCGAATT-3'). QRT-PCR and western blotting assays 48 hours post transfection were used to detect the efficiency of IGFBP3 siRNA and c-Jun siRNA. The following shRNAs were used: Control shRNA oligonucleotide, 5'-TTCTCCGAACGTGTCACGTTT-3'; IGFBP3 shRNA oligonucleotide, 5'-CAGAGCACAGATACCCAGAACTTCT-3'.
Zhang X., Wang G., Gong Y., Zhao L., Song P., Zhang H., Zhang Y., Ju H., Wang X., Wang B., Ren H., Zhu X, & Dong Y. (2023). IGFBP3 induced by the TGF-β/EGFRvIII transactivation contributes to the malignant phenotype of glioblastoma. iScience, 26(5), 106639.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!