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C ets 1

Manufactured by Santa Cruz Biotechnology
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C-Ets-1 is a transcription factor that regulates gene expression. It plays a role in cellular processes such as growth, differentiation, and development.

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Lab products found in correlation

Total h3, by Merck Group (2 mentions) Chip assay kit, by Merck Group (2 mentions) H3k4me3, by Merck Group (2 mentions) Control rabbit igg, by Santa Cruz Biotechnology (2 mentions) Stat3, by Santa Cruz Biotechnology (2 mentions)

Close spelling variants of this product

C-Ets-1 antibodies (C-20),

2 protocols using c ets 1

1

Regulation of Ube2n Gene Expression

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Bone marrow-derived macrophages were stimulated with IL-6 or IL-10, as indicated in the Figure legends. ChIPs were performed with antibodies to STAT3 (Santa Cruz), H3K4me3 (Millipore), total H3 (Millipore), Set1 (Bethyl Laboratories), c-Ets-1 (Santa Cruz) or rabbit IgG control (Santa Cruz) using a ChIP assay kit, per the manufacturer’s instructions (Millipore). For STAT3, Set1 or c-Ets-1, ChIP results were normalized first to the relevant input sample, and then to the untreated (NT) sample from STAT3-sufficient cells to determine fold change. For H3K4me3 ChIPs, results were normalized to data from ChIPs with total H3 antibody. Normalized data was plotted versus the corresponding predicted ChIP amplification product from the Ube2n proximal promoter region, as indicated by the schematic diagram in Figure 5.
Zhang H., Hu H., Greeley N., Jin J., Matthews A.J., Ohashi E., Caetano M.S., Li H.S., Wu X., Mandal P.K., McMurray J.S., Moghaddam S.J., Sun S.C, & Watowich S.S. (2014). STAT3 restrains RANK- and TLR4-mediated signaling by suppressing expression of the E2 ubiquitin ligase Ubc13. Nature communications, 5, 5798.
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2

Regulation of Ube2n Gene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bone marrow-derived macrophages were stimulated with IL-6 or IL-10, as indicated in the Figure legends. ChIPs were performed with antibodies to STAT3 (Santa Cruz), H3K4me3 (Millipore), total H3 (Millipore), Set1 (Bethyl Laboratories), c-Ets-1 (Santa Cruz) or rabbit IgG control (Santa Cruz) using a ChIP assay kit, per the manufacturer’s instructions (Millipore). For STAT3, Set1 or c-Ets-1, ChIP results were normalized first to the relevant input sample, and then to the untreated (NT) sample from STAT3-sufficient cells to determine fold change. For H3K4me3 ChIPs, results were normalized to data from ChIPs with total H3 antibody. Normalized data was plotted versus the corresponding predicted ChIP amplification product from the Ube2n proximal promoter region, as indicated by the schematic diagram in Figure 5.
Zhang H., Hu H., Greeley N., Jin J., Matthews A.J., Ohashi E., Caetano M.S., Li H.S., Wu X., Mandal P.K., McMurray J.S., Moghaddam S.J., Sun S.C, & Watowich S.S. (2014). STAT3 restrains RANK- and TLR4-mediated signaling by suppressing expression of the E2 ubiquitin ligase Ubc13. Nature communications, 5, 5798.
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