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Pbind yap1

Manufactured by Promega
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PBIND-YAP1 is a molecular biology product designed for use in scientific research. It is a plasmid construct that contains the YAP1 gene, which is a key regulator of the Hippo signaling pathway. This product is intended for research purposes only and its core function is to provide a tool for studying the role of YAP1 in cellular processes.

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Lab products found in correlation

Pflag cmv 2, by Merck Group (1 mentions) Pcw cas9, by Addgene (1 mentions) Pbind, by Promega (1 mentions) Pecfp c1, by Takara Bio (1 mentions) Glo lysis buffer, by Promega (1 mentions) Pg5luc, by Promega (1 mentions) Rnaimax, by Thermo Fisher Scientific (1 mentions) Lipofectamine ltx, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

PBIND

2 protocols using pbind yap1

1

Plasmid Construction for Estrogen Receptor and YAP1 Study

Cited 1 time
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pCW-FLAG-WT, Y537S, or D538G were created from pCW-Cas9 [62 (link)] (Addgene). pFLAG-CMV constructs were made from pFLAG-CMV2 (Sigma). YFP-tagged ESR1-YAP1 was constructed by PCR of the ESR1-YAP1 open reading frame (ORF) [11 (link)], followed by ligation into digested pECFP-C1 (Clontech). pBIND-YAP1 (GAL4 DBD-YAP1 amino acid 230–504 fusion expression plasmid) was constructed by ligation into pBIND (Promega). All constructs were sequenced.
Gates L.A., Gu G., Chen Y., Rohira A.D., Lei J.T., Hamilton R.A., Yu Y., Lonard D.M., Wang J., Wang S.P., Edwards D.G., Lavere P.F., Shao J., Yi P., Jain A., Jung S.Y., Malovannaya A., Li S., Shao J., Roeder R.G., Ellis M.J., Qin J., Fuqua S.A., O’Malley B.W, & Foulds C.E. (2018). Proteomic profiling identifies key coactivators utilized by mutant ERα proteins as potential new therapeutic targets. Oncogene, 37(33), 4581-4598.
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2

ERE-Luc and GAL4-Luc Assays in HeLa Cells

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For ERE-Luc assays, HeLa cells were transfected with pERE-E1b-Luc [39 (link)] and ERα expression vector using Lipofectamine LTX (Invitrogen), and lysed in Glo Lysis Buffer (Promega). For GAL4-Luc assays, cells were transfected with pBIND-YAP1 and pG5luc (Promega). Luc activity was measured on a Berthold luminometer as described [64 (link)]. Cells were co-transfected with pERE-E1b-luc, ERα expression plasmids, and siRNAs using Trans-IT-TKO (Mirus Corp.) or RNAimax (Invitrogen). siRNAs are listed in Supplementary Table 3.
Gates L.A., Gu G., Chen Y., Rohira A.D., Lei J.T., Hamilton R.A., Yu Y., Lonard D.M., Wang J., Wang S.P., Edwards D.G., Lavere P.F., Shao J., Yi P., Jain A., Jung S.Y., Malovannaya A., Li S., Shao J., Roeder R.G., Ellis M.J., Qin J., Fuqua S.A., O’Malley B.W, & Foulds C.E. (2018). Proteomic profiling identifies key coactivators utilized by mutant ERα proteins as potential new therapeutic targets. Oncogene, 37(33), 4581-4598.
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