Shim pack giss c18 column

The mass spectrometer (Shimadzu) with electrospray ionization mode (ESI) coupled to the HPLC Prominence (Shimadzu) was applied to record all mass spectra. The compounds were separated on a 50 × 2.1 mm i.d., 1.9 μm Shim Pack GISS C18 column (Shimadzu) thermostated at 40 °C. Samples were dosed in a volume of 2 μL and the flow rate was 0.2 mL/min. The separation of the analytes was performed in the same gradient systems as in the case of LC-DAD-ESI-MS/MS. The parameters of LCMS-IT-TOF spectrometer were set as follows: curved desolvation line (CDL) and heat block temperature was 230 °C, the nebulizing gas flow rate was 1.5 L/min, and capillary voltage was 4.5 kV. Positive ion mode with the mass range within 100–2000 Da was applied for recording all mass spectra. The collision energy was in the range of 12–50% independence on the structure of the compounds. The Formula Predictor within LCMS Solution software was used for the elaboration of results obtained in high-resolution mass spectrometry experiments (HRMS). Only an empirical formula with a mass error below 5 ppm was considered.

The mass spectrometer (Shimadzu) with electrospray ionization method (ESI) was applied to record all mass spectra. It was coupled to the HPLC Prominence (Shimadzu). Compounds were separated on a 50 mm × 2.1 mm i.d., 1.9 μm Shim Pack GISS C18 column (Shimadzu) thermostated at 40 °C. Samples were dosed in a volume of 2 μL and the flow rate was 0.2 mL/min. The separation of the analytes was performed in the same gradient systems as in the case of LC-DAD-ESI-MS/MS. Parameters of LCMS-IT-TOF spectrometer were set as follows: curved desolvation line (CDL) and heat block temperature 230 °C, nebulizing gas flow rate 1.5 L/min and capillary voltage 4.5 kV. Positive ion mode with mass range within 100–2000 Da was applied for recording all mass spectra. Collision energy was in the range of 12–50% depending on the structure of compounds. The Formula Predictor within the LCMS Solution software was used for elaboration of results obtained in high resolution mass spectrometry experiments (HRMS). Only empirical formula with a mass error below 5 ppm were taken into account.