Cyclophosphamide, etoposide, ifosfamide, and epirubicin were obtained from Macklin Biochemical Co., Ltd. (Shanghai, China), and daunorubicin, pirarubicin, pemetrexed, cytarabine, homoharringtonine, gemcitabine, irinotecan, paclitaxel, doxorubicin, docetaxel, idarubicin, raltitrexed, and methotrexate were obtained from Meilun Biotechnology Co., Ltd. (Dalian, China). Methanol and acetonitrile were purchased from Merck KGaA (Darmstadt, Germany), and HPLC-grade formic acid (99%) was purchased from Anaqua Chemicals Supply (Wilmington, USA). Purified water was obtained from a Merck Milli-Q HR Water Purification System (Darmstadt, Germany).
Gemcitabine
Manufactured by Meilun
Sourced in China
Gemcitabine is a chemotherapeutic agent used in the treatment of various types of cancer. It is a synthetic pyrimidine nucleoside analog that inhibits DNA synthesis and cell division. Gemcitabine is commonly used in combination with other chemotherapeutic agents or as a single agent for the management of solid tumors.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Docetaxel, by Meilun (1 mentions)
Cyclophosphamide, by Maclin Biochemical Technology (1 mentions)
Etoposide, by Maclin Biochemical Technology (1 mentions)
Epirubicin, by Maclin Biochemical Technology (1 mentions)
Daunorubicin, by Meilun (1 mentions)
Paclitaxel, by Meilun (1 mentions)
Irinotecan, by Meilun (1 mentions)
Doxorubicin, by Meilun (1 mentions)
Methotrexate, by Meilun (1 mentions)
Rabbit anti ki67, by Abcam (1 mentions)
Bcl 2, by Abcam (1 mentions)
Edta free trypsin, by Beyotime (1 mentions)
Microplate reader, by Bio-Rad (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Panc 1, by National Collection of Authenticated Cell Cultures (1 mentions)
Bxpc 3, by National Collection of Authenticated Cell Cultures (1 mentions)
Dulbecco modified eagle medium (dmem), by Corning (1 mentions)
4 protocols using gemcitabine
1
Anticancer Drug Compounds Sourcing
2
Wogonin and Gemcitabine: Cytotoxic Synergy
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Wogonin (Cat. MB6663-20 mg) was purchased from Meilunbio (Dalian, China); Gemcitabine (Cat. PHR2582-50 mg) was purchased from Sigma Aldrich (St Louis, MO, United States); EDTA-free trypsin (C0207) was purchased from Beyotime Biotechnology (Shanghai, China); the anti-β-actin antibody (Cat. 66009-1-lg) was purchased from Proteintech (Rosemont, IL, United States) and antibodies against pAKT (Thr) antibody (Cat.ab131474), Akt (Cat.ab8805), BAD (Cat.ab32455) and Bcl-2 (Cat.ab32124) were purchased from Abcam (Massachusetts, United States). The anti-rabbit Ki67 (Cat. ab15580) was also purchased from Abcam.
3
Gemcitabine Cytotoxicity Assay in Pancreatic Cancer Cell Lines
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Cells were collected and resuspended to a final concentration of 104 cells/ml in separate BxPC-M8 and BxPC-3-labeled plates respectively. Aliquots of the cell suspension were evenly distributed into 96-well plates. After incubating for 24 h at 37°C, the designated columns were treated with gemcitabine (Meilunbio) at concentrations 0, 0.2, 0.5, 1, 2, 5, 10, 20, 50 nM and incubated for 72 h at 37°C. MTT was added 4 h prior to analysusis. Formazan, the metabolite of MTT was dissolved with 150 μl dimethyl sulfoxide in each well. After 4 h of incubation at 37°C, all aliquots were discarded. The absorbance in individual wells was determined at 570 nm using a microplate reader (Bio-Rad Laboratories, Inc.).
4
Cytotoxicity Evaluation of Anticancer Compounds
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The human pancreatic adenocarcinoma PANC-1 and BxPC3 cell lines were purchased from the Cell Bank of the Chinese Academy of Science (Shanghai, China) and cultured in DMEM (Corning, Beijing, China) supplemented with 10% FBS (Sigma, Shanghai, China) and 1% Penicillin–Streptomycin (Sigma, Shanghai, China) at 37 °C with 5% CO2. In the exponential phase of the growth, cells were plated onto 96-well plates at a concentration of 8000 cells/well for 24 h. Compounds 1 –5 were prepared to various concentrations (80, 40, 20, 10, 5, 2.5, 1.25, and 0.625 µM in medium containing less than 0.1% DMSO) and incubated in 96-well plates (each concentration in six-fold wells) for 72 h. Gemcitabine (Gem, Meilunbio, ≥98%, Dalian, China) was offered as the positive control. Cell viability was determined according to reported assay methods using the commercial CCK8 kit (Elabscience, Wuhan, China) [29 (link)]. The optical density (OD) of each well was measured with an AMR-100 microplate reader at 450 nm (Allsheng Corporation, Hangzhou, China). Cytotoxicity emerged as the value of the drug concentration at the inhibition of cell growth by 50% (IC50).
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